白芍总苷对糖尿病大鼠肾组织p38 MAPK磷酸化与NF-κB表达的影响  

Effect of total glucosides of paeony on expression of phosphorylation of p38 mitogen-activated protein kinase and nuclear factor-κB in the kidney from diabetic rats

作　　者：张晶晶[1] 吴永贵[1] 张培[1] 苏静[1] 任克军[1] 齐向明[1] 张炜[1] 

机构地区：[1]安徽医科大学第一附属医院肾脏内科,合肥230022

出　　处：《安徽医科大学学报》2008年第5期534-537,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金资助项目(编号:070413100);安徽省教育厅自然科学基金资助项目(编号:2006KJ316B)

摘　　要：目的探讨白芍总苷(TGP)对糖尿病大鼠肾组织中p38MAPK磷酸化及NF-κBp65表达的影响。方法采用链脲佐菌素(STZ)诱导大鼠糖尿病模型。随机分为对照组、模型组、TGP组(50、100、200mg/(kg.d)),8周后应用West-ern免疫印迹方法检测肾组织p38MAPK磷酸化(p-p38)及NF-κBp65表达。结果TGP给药(50、100与200mg/kg)8周可明显降低大鼠尿白蛋白排泄。TGP给药(50mg/kg)大鼠肾小球平均容量(VG)明显低于模型组,肾小管一间质损伤指数(TⅡ)较模型组有所下降,但差异无显著性;TGP给药(100、200mg/kg)VG与TⅡ均明显低于模型组。Western印迹显示模型组肾组织p38MAPK磷酸化及NF-κBp65表达显著增强,TGP给药(50、100与200mg/kg)8周肾组织p38MAPK磷酸化表达下降30.8%、24.0%、60.1%,NF-κBp65表达下降27.8%、45.9%、59.0%。结论TGP可明显改善糖尿病大鼠早期肾损害,其机制可能与部分抑制糖尿病大鼠肾组织p38MAPK和NF-κB激活有关。Objective To investigate the effect of Total glucosides of paeony（TGP）on activation of p38 mitogen-activated protein kinase（p38 MAPK）and nuclear factor-κB p65（NF-κB p65）in the kidney from experimental diabetic rat.Methods Diabetes was induced with streptozotocin in rats,and TGP（50,100,200 mg/（kg·d））was orally administered once a day for 8 weeks.The expression of phosphorylation of p38（p-p38）MAPK and NF-κB p65 were determined by western blot analysis in the kidney.Results Elevated 24 h urinary albumin excretion rate was markedly attenuated by TGP treatment with 50,100 and 200 mg/kg.Elevated glomerular volume was significantly attenuated by TGP treatment with 50,100 and 200 mg/kg,and increased indices for tubulointerstitial injury were only ameliorated by TGP treatment with 100 and 200 mg/kg.Densitometric analysis of the Western blot showed increase in the amount of p-p38 and NF-κB p65 in the kidney from diabetic rats with respect to normal rats,TGP treatment with 50,100 and 200 mg/kg could reduced p-p38 and NF-κB p65 expression by 30.8%,24.0%,60.1% and 27.8%,45.9%,59.0%,respectively.Conclusion Our data suggest that TGP treatment ameliorates early renal injury via the inhibition of the overexpression of p-p38 and NF-κB p65 protein in the kidney from diabetic rats.

关 键 词：糖尿病肾病/药物疗法 丝裂原激活蛋白激酶类 

分 类 号：R282.710.7[医药卫生—中药学] R284.1[医药卫生—中医学]