Phylogenetic relationship and morphological evolution in the subfamily Limenitidinae(Lepidoptera:Nymphalidae)  

作　　者：Min Zhang Yang Zhong Tianwen Cao Yupeng Geng Yuan Zhang Ke Jin Zhumei Ren Rui Zhang Yaping Guo Enbo Ma 

机构地区：[1]Research Institute of Applied Biology and College of Life Science and Technology, Shanxi University, Taiyuan, Shanxi 030006, China [2]School of Life Sciences, Fudan University, Shanghai 200433, China [3]Institute of Plant Protection, Shanxi Academy of Agricultural Science, Taiyuan 030031, China

出　　处：《Progress in Natural Science:Materials International》2008年第11期1357-1364,共8页自然科学进展·国际材料（英文版）

基　　金：supported by National Natural Science Foundation of China(30570247 and 30670361);the Natural Science Foundation of Shanxi Province(2006011075);National Infrastructure of Natural Resources for Science and Technology(2005DKA21403);National Talent Training Fund in Basic Research of China(J0630643)

摘　　要：The mitochondrial cytochrome oxidase subunit I （CO1） gene and the nuclear elongation factor 1 α（EF-1α） gene were sequenced from 29 species of Nymphalidae （Nymphalidae, Lepidoptera）. Phylogenetic trees were constructed based on the sequences determined from the 29 species and sequences of other 36 species deposited in GenBank using the neighbor-joining （NJ）, maximum likelihood （ML） and Bayesian methods with Libythea celtis （Libytheinae） as the outgroup. Our phylogenetic trees indicated four major clades. Clade A includes three subfamilies： Apaturinae, Nymphalinae, and Limenitidinae, excluding the tribe Limenitidini; Clade B includes the subfamilies Heliconiinae and the tribe Limenitidini; Clade C includes Satyrinae, Calinaginae, Charaxinae and Morphinae; and Clade D includes subfamily Danainae. Our study suggested that the tribes Pseudergolini, Biblidini, Limenitidini and Cyrestidini should be considered as subfamilies and confirmed the interspecific relationships within the subfamily Pseudergolinae, namely Amnosia ＋（Pseudergolis ＋ （Stibochiona ＋ Dichorragia））. We then mapped three morphological characters （spot of anal angle, eyespots, and process from outer margin of hind wing） onto the phylogenetic tree constructed by ML analysis using the combined sequence data. Based on this the evolutionary patterns of these morphological characters were identified, they indicated that the three characters evolved repeatedly in the family Nymphalidae.mitochondrial 细胞色素氧子单元我(COI ) 基因和原子延伸因子 1 α(EF-1 α) 基因从 Nymphalidae 的 29 种类被定序(Nymphalidae，鳞翅目) 。种系发生的树基于从 29 种类决定的序列和在 GenBank 扔用的另外的 36 种类的序列被构造加入邻居(NJ ) ，最大的可能性(ML ) 和有 Libythea celtis (Libytheinae ) 的贝叶斯的方法作为外集团。我们的种系发生的树显示了四主要 clades。Clade A 包括三个亚科：Apaturinae， Nymphalinae，和 Limenitidinae，排除部落 Limenitidini；Clade B 包括亚科 Heliconiinae 和部落 Limenitidini；Clade C 包括 Satyrinae， Calinaginae， Charaxinae 和 Morphinae；并且 Clade D 包括亚科 Danainae。我们的学习建议部落 Pseudergolini， Biblidini， Limenitidini 和 Cyrestidini 应该被看作亚科并且在亚科 Pseudergolinae 以内证实了种间的关系，也就是 Amnosia +(Pseudergolis +(Stibochiona + Dichorragia )) 。我们然后印射三个词法字符(从后部的翅膀的外部边缘的肛门角度，视觉器官，和过程的点) 到 ML 分析用联合顺序数据构造的种系发生的树上。把这些词法人物的进化模式基于这被识别，他们显示三个人物在家庭 Nymphalidae 反复演变。

关 键 词：NYMPHALIDAE Limenitidinae Mitochondrial DNA cytochrome oxidase subunit I gene （CO1） Nuclear elongation factor 1α gene （EF-1α） Molecular phylogeny Character evolution 

分 类 号：Q969.42[生物学—昆虫学]