GFAP与α-SMA在涎腺腺样囊性癌中共表达的免疫电镜研究  

Coexpression of GFAP andα-SMA in salivary adenoid cystic carcinoma: an immunoelectron microscopic analysis

作　　者：吕成鹏[1] 孙沫逸[1] 陈伟[1] 李建虎[1] 黄晓峰[2] 于华[2] 王春梅[2] 

机构地区：[1]第四军医大学口腔医学院颌面外科,陕西西安710032 [2]第四军医大学电镜教研室,陕西西安710032

出　　处：《临床口腔医学杂志》2008年第10期581-583,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(30271424);国家自然科学基金资助(30772428);陕西省自然科学基金资助(2002C2-06)

摘　　要：目的:研究雪旺氏细胞标志物胶原原纤维酸性蛋白(GFAP)和肌上皮细胞标记物α-平滑肌肌动蛋白(α-SMA)在涎腺腺样囊性癌(ACC)中的共表达情况,探讨GFAP、α-SMA与ACC嗜神经侵袭的关系。方法:利用双重胶体金标记的免疫电镜技术从超微结构水平研究GFAP和α-SMA在腺样囊性癌中的共表达。结果:在ACC组织中,GFAP蛋白与α-SMA蛋白均有表达,GFAP和α-SMA共表达的细胞符合肌上皮细胞的超微结构特点。结论:ACC中肿瘤性肌上皮细胞样发生雪旺氏细胞分化可能是ACC嗜神经侵袭的组织病理学基础。Objective： To explore the expression of Schwann cell marker GFAP and myoepithelial cell marker α-SMA in salivary adenoid cystic carcinoma （ACC）, and to evaluate the relationship of GFAP, α-SMA and perineural invasion in ACC. Method： an immunoelectron microscopic of double labeling colloidal gold method was used to detect the expression of GFAP and α-SMA in salivary ACC tissue samples.Result： In salivary ACC tissue samples, both GFAP and α-SMA proteins were positive.The cell in which GFAP and α-SMA coexpressed accorded with the characteristics of myoepithelial cells. Conclusion：There may be Schwann cell differentitation in onco-myoepithelial cell of salivary ACC,and it may be the pathological base of perineural invasion in salivary ACC.

关 键 词：腺样囊性癌 胶原原纤维酸性蛋白 Α-平滑肌肌动蛋白 双重胶体金标记 免疫电镜 

分 类 号：Q786[生物学—分子生物学]