CD5^+B细胞与扩张型心肌病相关性研究  

Relationship between CD5^+ B cells and dilated cardiomyopathy

作　　者：袁璟[1] 廖玉华[1] 张丽华[1] 程翔[1] 王敏[1] 郭和平[1] 

机构地区：[1]华中科技大学协和医院心内科,华中科技大学同济医学院心血管病研究所,湖北省生物靶向治疗研究重点实验室,武汉430022

出　　处：《临床心血管病杂志》2008年第9期653-655,共3页Journal of Clinical Cardiology

基　　金：国家重点基础研究发展计划(973计划)资助(No:2007CB512005);湖北省生物靶向治疗研究重点实验室开放基金项目(No:2007A01)

摘　　要：目的:通过对扩张型心肌病(DCM)患者体内CD5+B细胞及其功能的检测,探讨它在DCM发生发展中的作用。方法:选择DCM患者42例,20例健康者为对照。采用双色流式细胞术检测患者外周血淋巴细胞中CD5+B细胞(CD19+CD5+)数,ELISA法检测血清抗心肌自身抗体(AHA)以及体外CD5+B细胞分泌IL-10功能;根据彩色多普勒检测心脏射血分数(EF)。结果:与对照组[(5.39±1.46)%]相比,DCM患者CD5+B细胞均有明显增多,其中AHA阳性者为(13.49±3.15)%,AHA阴性者(14.06±5.23)%,AHA阳性和阴性者CD5+B细胞差异无统计学意义。对照组AHA检测均为阴性。DCM患者CD5+B细胞的增多与心脏EF值无相关性(r=-0.25,P>0.05),但其分泌IL-10功能与EF值负相关(r=-0.64,P<0.01)。结论:CD5+B细胞不仅参与AHA阳性也参与AHA阴性DCM发病过程;有活性的CD5+B细胞介导DCM心力衰竭的进程。Objective： To explore the role of CD5^＋B cells in the development of dilated cardiomyopathy （DCM） by investigating the number and function of CD5^＋B cells from patients with DCM. Method： Forty-two patients with DCM were recruited to be studied and twenty healthy people as controls. Two-color Flow cytometry was used to investigate the percentage of blood CD5^＋B cells （CD19^＋ CD5^＋ ）, while the sera anti-heart autoantibodies （AHA） and the IL-10 secreted from CD5^＋B cells in vitro were measured with ELISA. The data of the heart ejection fraction （EF） were obtained by ultrasonic cardiogram. Result： Compared with the controls [ （5.39 ±1.46）], the percentages of CD5^＋B cells were all markedly increased in DCM group, with the positive AHA（13.49±3.15） %, and negative AHA（14. 06±5. 23） %. No significant difference was found in the levels of CD5^＋B cells between positive AHA patients and negative ones. The AHA was not found in the controls. The capability of secretion of IL-10 from CD5^＋B cells was negatively correlated with the EF value of patients with DCM（γ=-0.64, P〈0.01） but not correlated with the number of CD5^＋B cells （γ= -0.25 ,P〉0. 05）. Conclusion：CD5^＋B cells are involved in the pathogenesis of DCM either with positive AHA or with negative one. And not the increased number but the activation of CD5^＋B cells mediates the development of the heart failure in DCM.

关 键 词：扩张型心肌病 CD5 B细胞 自身抗体 心力衰竭 

分 类 号：R542.2[医药卫生—心血管疾病]