运动对1型糖尿病大鼠心肌肌浆网钙调控蛋白表达的影响  

Effect of exercise on gene expression of calcium modulin in myocardial sarcoplasmic reticulum of diabetic rat

作　　者：江卫华[1,2] 罗达亚[1] 余乐涵[1] 段荣[2] 万福生[1] 

机构地区：[1]南昌大学医学院生物化学与分子生物学教研室,南昌330006 [2]江西省儿童医院检验科

出　　处：《中华物理医学与康复杂志》2008年第10期653-657,共5页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察运动对1型糖尿病大鼠心肌肌浆网钙调控蛋白表达的影响，并探讨其机制。方法选用健康Sprague—Dawley大鼠，随机分为正常对照组、运动对照组、糖尿病组和糖尿病＋运动组，每组10只。用腹腔注射链脲佐菌素（55mg／kg）法复制糖尿病模型，糖尿病＋运动组大鼠在糖尿病模型建成后第4天开始跑台运动，于运动第4周末心脏采血，制备血清。采用放射免疫法测定血清胰岛素水平，采用RT—PCR法检测肌浆网Ca^2＋-ATP酶（SERCA）、磷酸受纳蛋白（PLB）和Ryanodine受体-2（RyR2）mRNA的表达，采用Western—blotting法检测SERCA2、PLB蛋白的表达。结果与正常对照组相比，糖尿病组血糖、糖化血清蛋白及低密度脂蛋白水平升高，胰岛素和高密度脂蛋白水平降低，心肌SERCA2、PLB、RyR2mRNA和SERCA2、PLB蛋白表达差异无统计学意义；糖尿病＋运动组血糖水平升高，胰岛素水平降低，心肌SERCA2、PLB和RyR2的mRNA表达水平提高，SERCA2、PLB蛋白表达量增加，差异有统计学意义（P〈0．01）。与糖尿病组相比，糖尿病＋运动组大鼠糖化血清蛋白及低密度脂蛋白水平显著降低（P〈0．01），SERCA2、PLB、RyR2mRNA及SERCA2、PLB蛋白表达显著升高（P〈0．01）。结论运动对1型糖尿病大鼠心肌损伤有防治作用，其机制可能与运动上调SERCA2、PLB和RyR2的表达有关。Objective To investigate the effect of exercise on calcium modulin in myocardial sarcoplasmic reticulum of animal type 1 diabetes model in rat. Methods A total of 40 Sprague-Dawley rats were randomly divided into 4 groups ： a normal control group, an exercise training group, a diabetes group and a diabetes plus exercise-training group. At the end of 4- week-exercise training after the establishment of the diabetes model by intraperitoneal injection of sterptozotocin, the animals were sacrificed and the level of blood glucose, insulin, blood fat and glycosylated serum protein were tested. The gene expression of calcium modulin proteins was measured by reverse transcription-polymerase chain reaction, and the Western blotting technique was used to measure the protein of sarcoplasmic endoplasmic reticulum Ca^2＋-ATPase （SERCA2） and phospholamban （PLB）. Results The level of biochemical indicator of exercise group is not affected when comparing with that of the control group, but significantly changed in diabetic group （P 〈 0.01 ） ; The level of blood glucose, insulin, blood fat and glycosylated serum protein were ameliorated in diabetic rats in the exercise training group. No significant changes in mRNA level of SERCA2, PLB and ryanodine receptor type 2 （RYR2） were observed between control and diabetic group, the same to protein expression of SERCA2 and PLB. But expression of calcium modulin mRNA was significantly increased in exercise group and diabetic rats in the exercise training group comparing with that of the control and diabetic groups （P 〈 0.01 ）, the same to protein expression of SERCA2 and PLB. Conclusion Exercise exerted good protective effects on the myocardial injury with 1 type diabetes rat, which might attribute to the upregulated expression of SERCA2, PLB and RYR2 in diabetic rat heart.

关 键 词：运动 糖尿病 钙调控蛋白 肌浆网 磷酸受纳蛋白 Ryanodine受体-2 

分 类 号：R455[医药卫生—运动医学] R587.1[医药卫生—治疗学]