豚鼠耳蜗外毛细胞的简化分离  

A simple method of separating outer hair cells in guinea pig

作　　者：康喜讯[1] 孔维佳[1] 黎万荣[2] 曾晓荣[3] 杨艳[3] 张松[1] 郭长凯[1] 

机构地区：[1]华中科技大学同济医学院协和医院耳鼻咽喉科,武汉430022 [2]泸州医学院附属医院耳鼻咽喉-头颈外科 [3]泸州医学院心肌电生理研究所

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第18期829-831,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:为获得能够满足电生理需要的外毛细胞,探讨简单可靠的分离外毛细胞的方法。方法:暴露听泡后去除耳蜗骨壳,将螺旋韧带连同基底膜和蜗轴一起放入酶液消化,减少显微镜下分离基底膜的操作程序,同时避免去除螺旋韧带时部分基底膜片段的丢失。结果:获得较多的保持良好活性的单离外毛细胞,耳大约80%的细胞能保持活性平均约6h。结论:去除骨壳后的残余耳蜗整体消化并适当提高消化液浓度的分离法,操作简单,可以获得能够满足电生理实验需要的较多的单离的耳蜗外毛细胞,有利于内耳研究工作的开展。Objective：In order to meet the needs of electrophysiology outer hair cells ,we investigate a method to separate outer hair cells simply and effectively. Method：The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette. Result：A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours. Conclusion：If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymed, we can get living outer hair cells easily.

关 键 词：外毛细胞 分离 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]