单侧迷路切除术后毒蕈碱受体亚型在大鼠小脑绒球中的变化  

Expression change of Muscarinic receptor subnuits in rat flocculus following unilateral labyrinthectomy

作　　者：邹宇[1,2] 孔维佳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022 [2]广州市儿童医院耳鼻咽喉科,广州510120

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第19期896-898,903,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家杰出青年基金(No:39925035);卫生部临床学科重点项目基金(No:2004468)

摘　　要：目的：观察左侧迷路切除术后大鼠小脑绒球内毒蕈碱受体M1、M3、M5亚型的表达变化。方法：利用RT-PCR的方法,切除大鼠一侧迷路,观察小脑绒球毒蕈碱受体M1、M3、M5亚型表达的变化,及其在前庭代偿中可能的作用。结果：单侧迷路切除术（UL）后可诱导双侧绒球内毒蕈碱受体M1、M3、M5亚型减少,术后1 d最少,此后3-7 d处于上升趋势,7 d时和假手术组比较表达差异无统计学意义。各组手术两侧比较均差异无统计学意义。结论：UL后可诱导绒球内毒蕈碱受体M1、M3、M5亚型减少,但其在前庭代偿中的作用尚有待研究。Objective：To observe the expression of Muscarinic receptor M1,M3,M5 subunits in rat flocculus following left unilateral labyrinthectomy（UL）.Method：The RT-PCR was used to observe the expression of Muscarinic receptor M1,M3,M5 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation.Result：Muscarinic receptor M1,M3,M5 subunits were induced decrease in both side flocculus after unilateral labyrinthectomy.The expression was the least in the 1 d flocculus of following UL.The expression is rising from the 3～7 d flocculus of following UL.No difference was observed in the 7 d and sham operation flocculus following UL.No difference was observed in the ipsilateral and contralateral flocculus at any group.Conclusion：Muscarinic receptor M1,M3,M5 subunits were induced decrease in the flocculus after unilateral labyrinthectomy.But the significance of the change of Muscarinic receptor M1,M3,M5 subunits in the vestibular compensation is still unknown.

关 键 词：绒球 前庭代偿 毒蕈碱受体 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]