BALB／c鼠和裸鼠呼吸道合胞病毒感染比较研究  

Infection of respiratory syncytial virus in BALB/c mice and nude mice

作　　者：周娟[1] 杨锡强[1] 赵晓东[1] 王莉佳[1] 蒋利萍[1] 

机构地区：[1]重庆医科大学附属儿童医院肾脏免疫科,400014

出　　处：《中华微生物学和免疫学杂志》2008年第10期909-913,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（30340045）

摘　　要：目的比较普通BALB／c鼠和裸鼠呼吸道合胞病毒（RSV）感染免疫及炎症反应特点。方法BALB／c鼠和裸鼠感染RSV后不同时间空斑形成试验检测肺组织病毒滴度，计数支气管肺泡灌洗液（BALF）白细胞总数和分类，HE染色分析肺组织病理学改变，免疫组化检测肺组织F4／80^＋细胞和CD9b^＋细胞。ELISA检测BALF中TNF—α、IFN-γ、IL-12和IL-10浓度。结果BALB／c鼠和裸鼠感染RSV后肺组织病毒滴度在第3天达峰值，感染裸鼠带毒时间更长，在感染后各天病毒滴度明显高于BALB／c鼠（P〈0．05），肺组织病理改变也更重。感染BALB／c鼠和裸鼠BALF白细胞总数明显升高，分类以淋巴细胞为主。感染裸鼠与感染BALB／c鼠比较，肺组织检测到更多的F4／80^＋巨噬细胞和CD49b^＋NK细胞（P〈0．05），BALF中TNF—α、IL-12和IL-10水平更高（P〈0．05）。结论RSV感染裸鼠与BALB／c鼠比较，病毒复制水平更高，时间更持久，炎症反应更重。单核巨噬细胞和NK细胞是RSV感染重要的免疫细胞和炎症细胞，炎症反应强度并不一定与T细胞免疫应答平行。Objective To compare respiratory syncytial virus （RSV） infection and inflammatory responses between immunocompetent BALB/c mice and immunodeficient nude mice. Methods At various time points after RSV infection of BALB/c mice and nude mice, pulmonary viral titers were assayed. Leukocytes in bronchoalveolar lavage fluid （BALF） and pulmonary histology were identified. F4/80^ ＋ cells and CD49b^＋ cells in lung tissue were examined by immunohistochemistry, and the cytokines of TNF-α, IL-12, IFN-γ and IL-10 in BALF were assayed by ELISA. Results RSV fiters in infected BALB/c mice and nude mice peaked on the 3rd day postinoculation, and nude mice had higher-level and more durative viral replication than BALB/c mice. RSV infection induced more severe pulmonary histopathology and larger number of leukocytes in airway in nude mice than in BALB/c mice. RSV infection enhanced more pulmonary F4/80^＋ macrophages, CD49b ^＋ NK cells in both mice. Furthermore infected nude mice had larger amount of pulmonary macrophages and NK cells than infected BALB/c mice. RSV infected BALB/c mice secreted more TNF-α, IL-12, IFN-γ and IL-10 as compared with control BALB/c mice, and infected nude mice had higher level of TNF-α, IL-12 and IL-10 than infected BALB/c mice. Conclusion Nude mice are good model for severe and persistent RSV infection in immunocomprised hosts. The inflammation induced by RSV infection is not parallel with the immune response of T cells, and macrophages and NK cells are potent immunocytes and inflammatory cells in RSV infection especially when T lymphocytes are absent.

关 键 词：呼吸道合胞病毒 炎症 免疫 

分 类 号：R5[医药卫生—内科学]