单侧迷路切除后γ-氨基丁酸A受体亚单位在大鼠小脑绒球中的表达  

The Change in the Expression of Gamma-Amino Butyric Acid A Receptor Subunits in Rat's Flocculus

作　　者：刘珺[1] 孔维佳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022

出　　处：《听力学及言语疾病杂志》2008年第6期491-494,共4页Journal of Audiology and Speech Pathology

基　　金：国家杰出青年基金(编号39925035);卫生部临床学科重点项目基金(编号2001321)资助

摘　　要：目的观察单侧迷路切除术后大鼠小脑绒球内γ-氨基丁酸A（gamma—amino butyric acid A，GABAA）受体α3、β1、δ亚单位的表达变化。方法成年雄性Wistar大鼠24只，随机分为迷路破坏组（18只）和对照组（6只），前者破坏单侧迷路，对照组手术方式相同但保持迷路完好。通过免疫组织化学法检测术后不同存活时间（术后1、3、7天）两组动物小脑绒球内GABAA受体α3、β1、δ亚单位的表达变化。结果单侧迷路切除术后大鼠术侧小脑绒球内GABAA受体β1、δ亚单位增加，术后1天最多，此后3～7天处于下降趋势，7天时和对照组比较表达无差异。α3亚单位在小脑绒球的染色很弱，各组两侧比较及术后1、3、7天与对照组比较均无明显差异。结论单侧迷路切除后大鼠术侧小脑绒球内GABAA受体β1、δ亚单位增加；双侧前庭中枢神经元的静息放电的平衡可能与GABAA受体β1、δ亚单位绒球内的增加有关；α3亚单位可能并非GABAA受体在小脑绒球主要的结构与功能证单侍。Objective To observe the expression of gamma--amino butyric acid A receptor （GABAA receptor） α3、β1、δ subunits in rat flocculus following unilateral labyrinthectomy （UL）. Methods 24 wistar rats were randomly divided into two groups. 18 animals received unilateral labyrinthectomy while the others maintained labyrinthine well. After removing left labyrinthine, the change of GABAA receptor α3、β1、δ subunits was detected by immunohistochemistry. Results GABAA receptor β1、δ subunits in flocculus was induced on the operated side after unilateral labyrinthectomy. The most expression was on the 1st day floeculus of following UL. The expression is descending from the 3rd day to 7th day flocculus of following UL. The GABAA receptor α3 subunit immunostaining in flocculus was weak and did not change in the process of vestibular compensation. Conclusion GABAA receptor 91,8 subunits were induced increase in the floceulus after unilateral labyrinthectomy. The alteration in the resting discharge of the central vestibular neurons may be caused by the increase of GABAA receptor β1、δ subunits in the flocculus. But the significance of the change of GABAA receptor β1、δ subunits in the vestibular compensation is still unknown. Our study suggests that GABAA receptor α3 subunits may not participate in constructing GABAA receptor in the floceulus.

关 键 词：绒球 前庭代偿 Γ-氨基丁酸A受体 大鼠 

分 类 号：R764.34[医药卫生—耳鼻咽喉科]