耳蜗缺血损伤动物模型的建立  

A Guinea Pig Model of Cochlear Ischemia

作　　者：冯彪[1] 邱建华[1] 

机构地区：[1]西安第四军医大学西京医院全军耳鼻咽喉头颈外科中心,西安710032

出　　处：《听力学及言语疾病杂志》2008年第6期502-505,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目(项目编号:39200133)

摘　　要：目的探讨建立一种耳蜗缺血动物模型的方法。方法将30只豚鼠随机分为3组：正常组、手术组、血栓组，每组10只。血栓组通过手术经颅底暴露小脑前下动脉（anterior inferio cerebellar artery，AICA），使用40％三氯化铁（FeCl3）溶液诱导AICA形成血栓；手术组仅完成暴露豚鼠小脑前下动脉；正常组未作特殊处理。手术完成后饲养动物1周，实验过程中采用激光多普勒血流量仪监测耳蜗底回血流量（cochleablood flow，CoBF），实验前后及术后1周测试三组豚鼠听性脑干反应（ABR）波Ⅲ反应阈，血栓组将AICA组织切片HE染色观察血栓形态。结果手术组和血栓组动物均存活7～30天，血栓组在FeCl3诱导后，CoBF值下降至诱导前约50％，ABR波Ⅲ反应阈均比实验前有明显提高，正常组和手术组实验前后差异无统计学意义。AICA切片HE染色，可见血栓组AICA有明显白色血栓形成，成分为纤维素、血小板及少量红细胞。结论应用FeCl3能够成功诱导小脑前下动脉血栓形成，可制备耳蜗缺血损伤模型；动物能够存活1周以上，最长达30天，是一种慢性耳蜗缺血损伤模型研究的新尝试，为临床研究慢性内耳微循环障碍疾病提供较理想的实验动物模型。Objective To explore a new method for establishing an animal model of cochlear ischemia. Methods Thirty guinea pigs were randomized into three groups： normal group, operation group, ischemia group. Anterior inferior eerebellar artery （AICA） was exposed surgically via a skull base approach. In ischemia group, a small piece of filter paper containing FeCla was placed upon the AICA to induce thrombus formation. In operation group,AICA was only exposed. In normal group, nothing was done to animal. The blood flow of cochlea（CoBF） was continuously monitored with laser Doppler flowmeter. The thresholds of auditory brainstem response （ABR） were measured before, after each experiment,and one week after operation. The AICAs of ischemia group and operation group were resected and pathological sections were perfomed for observation of the details. Results After the administration of FeCl3, the CoBF decreased to 50% of the base level at 30 min. In the AICA pathological sections of ischemia group , white thrombus composed of cellulose, blood platelets and erythroeytes could be seen. The threshold of ABR in ischemia group increased significantly. Conclusion The method provides a relatively ideal model for ischemis of the inner ear and this model may be helpful in studying the mechanisms of hearing loss caused by chronic microcirculatory disorders.

关 键 词：动物模型 慢性缺血 耳蜗 三氯化铁 动脉血栓 耳蜗血流量 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]