锰超氧化物歧化酶9Ala/Val基因多态性与冠心病的关系  

Association Between Manganese Superoxide Dismutase 9Ala/Val Genetic Polymorphisms and Coronary Heart Disease

作　　者：杨茂平[1] 叶林秀[2] 邱红[3] 邓德明[3] 王琴[3] 李巧林[3] 雷婷[3] 迟东升[4] 

机构地区：[1]东风汽车公司总医院老年科,湖北十堰4420004 [2]东风汽车公司总医院内分泌科,湖北十堰4420004 [3]东风汽车公司总医院检验科,湖北十堰4420004 [4]广东省中医院心脏中心,广东广州510105

出　　处：《武汉大学学报（医学版）》2008年第6期775-779,共5页Medical Journal of Wuhan University

基　　金：广东省自然科学基金团队项目(编号:015042);中国博士后科学基金(编号:2004036155)

摘　　要：目的:探讨锰超氧化物歧化酶9 Ala/Val(MnSOD9 Ala/Val)基因多态性与冠心病、血浆总超氧化物歧化酶(T-SOD)和锰超氧化物歧化酶(MnSOD)活性的关系。方法:采用ABI 3100 DNA测序仪检测147例冠心病患者和108例对照组的MnSOD9 Ala/Val基因多态性的基因型,采用比色法测定血浆T-SOD和MnSOD活性。结果:与对照组比较,冠心病患者的血浆T-SOD和MnSOD活性明显降低,C反应蛋白(CRP)和颈动脉内-中膜厚度(IMT)则显著增高;冠心病患者的MnSOD VV基因型和V等位基因携带者较对照组明显增多;MnSOD VV基因型的T-SOD和MnSOD活性较AA基因型明显降低,并且MnSOD 9V等位基因与T-SOD活力、MnSOD活力和高密度脂蛋白呈负相关;与空腹血糖、胆固醇、甘油三酯、低密度脂蛋白、收缩压、IMT和CRP呈正相关。结论:冠心病患者的抗氧化能力明显降低,因而炎症反应和动脉硬化程度加重;MnSOD 9 Ala/Val基因多态性通过影响血浆MnSOD活性和冠心病的危险因素而参与冠心病的发病。Objective： To study the associations between manganese superoxide dismutase 9Ala/Val （MnSOD 9 Ala/Val） genetic polymorphisms and coronary heart disease （CHD）. Methods： The ABI 3100 DNA sequencer was applied to identify the genotype of MnSOD 9 Ala/Val genetic poly- morphisms, and colorimeter was used to detect plasma activities of total superoxide dismutase （T-SOD） and MnSOD in 147 CHD patients and 108 controls. Results： Compared with that of controls, the plasma activity of T-SOD and MnSOD reduced obviously, while C-reaction protein （CRP） concentration and intima-medium thickness （IMT） in carotid artery increased markedly in CHD patients. There were more VV genotype and V allele of MnSOD 9 Ala/Val genetic polymorphisms in CHD patients than in controls. The activity of plasma T-SOD and MnSOD decreased, while CRP and IMT increased in individuals with MnSOD 9 VV genotype compared in those with AA genotype, and MnSOD 9V allele correlated negatively with the activity of T-SOD, MnSOD and serum HDL level, positively with fasting plasma glucose, total cholesterol, triglyceride, LDL, systolic blood pressure, IMT and CRP. Conclusion： The antioxidative ability decreased, while inflammatory reaction and degree of atherosclerosis increased in CHD patients. Gene polymorphisms of MnSOD 9 Ala/Val involves in the pathogenesis of CHD by influencing the plasma activities of T-SOD, MnSOD and risk factors of CHD.

关 键 词：冠心病 锰超氧化物歧化酶 基因 多态性 

分 类 号：R541.4[医药卫生—心血管疾病]