供体细胞的不同选择和处理对重编程过程的影响  

Effect of different choices and treatments with donor cells on reprogramming

作　　者：姚雅馨[1,2] 李向臣[1] 张勇[2] 乔利敏[1,2] 关伟军[1] 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]甘肃农业大学动物医学院,兰州730070

出　　处：《遗传》2008年第11期1392-1396,共5页Hereditas（Beijing)

基　　金：国家高技术研究发展计划项目(863计划)(编号:2006AA10Z198);国家自然科技平台建设项目(编号:2005DKA21101);国家“十一五”科技支撑计划(编号:2006BAD13B08)资助~~

摘　　要：供体细胞核移入去核的卵母细胞后,必定要经过表观遗传修饰的重编程过程,回到胚胎开始发育的全能状态。若重编程过程不完全,必定会导致克隆效率降低。但是,体细胞核的重编程能力不仅仅是在其移入去核的卵母细胞后所体现,它在不同的供体细胞当中的潜能也是不尽相同的。并且,对供体细胞进行不同的处理,也会导致其重编程的能力和程度不同。文章首先阐述了供体细胞的类型、代数、周期、年龄以及物种的不同选择对其核移植后重编程过程的不同影响,其后又通过对供体细胞的冷冻保存,血清饥饿以及不同试剂的处理等方面对重编程过程所起到的作用作出了概况性的论述与分析。The donor nucleus must experienced the epigenefic modification of the process reprogramming and went back to the initial state after the donor cell was injected into the oocytes. If the reprogramming is not completed, the efficiency of cloning will be reduced. However, reprogramming of nucleus muct was not only embodied in its ability after it was trans- ferred into the oocytes. It was different in the potential if the cell type was not identical. In addition, different treatment to the donor cells resulted in different ability and the level of reprogramming. This paper described different effects of the type, algebra, cycles, age, and species of the donor cells after nuclear transplantation on the reprogramming. An overview of the exposition and analysis through the donor cell cryopreservation, serum starvation, and different reagent treatments were discussed.

关 键 词：供体细胞 核移植 重编程 

分 类 号：Q813[生物学—生物工程]