郏县红牛ZAG基因多态性与生长性状的相关性  

Polymorphisms of ZAG gene with growth traits in Jiaxian red cattle

作　　者：郭义昆[1] 陈宏[1,2] 张宝[1] 潘传英[1] 张良志[1] 赵苗[1] 张存芳[1] 蓝贤勇[1] 王居强 

机构地区：[1]西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,徐州221116 [3]河南肉牛工程技术研究中心,郑州450003

出　　处：《遗传》2008年第11期1417-1420,共4页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30771544);国家高技术研究发展计划项目(863计划)(编号:2006AA10Z197);国家支撑计划(编号:2006BAD01A10-5);河南省杰出人才创新基金项目(编号:0521001900);西北农林科技大学拔尖人才支持计划项目资助~~

摘　　要：ZAG基因的功能主要是促进脂肪分解,减少脂肪含量。文章利用PCR-SSCP和DNA测序技术研究了145头郏县红牛ZAG基因编码区4个位点(Z1、Z2、Z3、Z4)的多态性,发现Z1、Z3、Z4位点存在SSCP多态。对不同SSCP带型的对应片段进行了测序分析,共发现6个新的SNP多态位点(C115T、A3257G、A4013G、T4027C、C4032T、T4120C)。Z3位点处于Hardy-Weinberg平衡状态,Z1、Z4位点处于非平衡状态。不同基因型与生长发育性状的相关性分析显示,Z4位点上,AC基因型个体的体斜长、胸围、管围、体重指标显著(P<0.05)或极显著(P<0.01),大于AA、AB基因型个体,暗示该位点有可能作为郏县红牛生长性状标记辅助选择的标记之一。The main function of ZAG gene is to enable the decomposition of fat, and reduced fat content. In this study, polymorphisms of four loci （Z1, Z2, Z3, Z4） at the coding region of the bovine ZAG gene were detected in 145 Jiaxian red cattle, and polymorphisms were found on Z1, Z3, Z4 loci. The fragments showing different SSCP patterns were sequenced, nd a total of six SNPs （C115T, A3257G, A4013G T4027C, C4032T, and T4120C） were found. The Z3 locus of ZAG gene was at Hardy-Weinberg equilibrium, while Z1 and Z4 loci of ZAG gene were not at Hardy-Weinberg disequilibrium. The association analysis of different genotypes with some of the growth and development traits showed that the individuals with genotype AC had a larger body length, heart girth, circumference of cannon bone, body weight than the individuals with genotypes AA and AB （P〈0.05 or P〈0.01, respectively） in Z4 locus. It is suggest that Z4 locus may be one of the marker-assisted selection used as growth traits in Jiaxian red cattle.

关 键 词：郏县红牛 ZAG基因 PCR-SSCP 生长发育性状 

分 类 号：S823[农业科学—畜牧学]