兔GHR基因单核苷酸多态性及其与屠宰性状的相关性  

The correlations between polymorphism of growth hormone receptor gene and butcher traits in rabbit

作　　者：邓小松[1] 万洁[1] 陈仕毅[1] 王彦[1] 赖松家[1] 蒋美山[1] 徐敏[1] 

机构地区：[1]四川农业大学动物科技学院,雅安625014

出　　处：《遗传》2008年第11期1427-1432,共6页Hereditas（Beijing)

基　　金：公益性行业(农业)科研专项经费资助(编号:nyhyzx07-040)~~

摘　　要：采用PCR-SSCP技术和DNA测序的方法,对比利时兔、天府黑兔、齐卡巨型兔、哈尔滨白兔以及加利福尼亚兔5个肉兔品种的生长激素受体(Growth hormone receptor,GHR)基因进行单核苷酸多态性分析。结果发现了2个单核苷酸多态位点,分别位于外显子10的705(T→C)和810(C→T)。通过最小二乘分析SNPs及其与屠宰性状的关系发现,基因型AA和MM所对应的活重、全净膛、屠宰率最小二乘均值都显著低于基因型BB和NN(P<0.05)。但各基因型饲料转化率最小二乘均值差异不显著(P>0.05)。由此可以初步推断,GHR基因是影响兔体重和屠宰率等屠宰性状的主要候选基因。Five rabbit populations （Belgian hare, Tianfu black rabbit, Great line of Zika rabbit, Harbin white rabbit, and California rabbit） were used to analyze the polymorphism of growth hormone receptor （GHR） gene by PCR-SSCP. Results indicated that there were two mutation sites （C705T and C810T） in the 5 populations. The least square analyses showed that the live weight, visceraste weight, and slaughter percentage of AA and MM genotypes were significandy lower than BB and NN genotypes （P〈0.05）. In contrast, the GHR polymorphism had no significant difference for least squares means of feed transformation efficiency （P〉0.05）. It suggested that GHR gene may be a candidate gene responsible for butcher trait in rabbit.

关 键 词：兔 生长激素受体基因 PCR-SSCP 屠宰性状 

分 类 号：S829.1[农业科学—畜牧学]