应激QTL座位22个微卫星标记与猪应激性状指标的关系  

Effects of 22 microsatellite DNA markers on stress traits in F_2 population of pigs

作　　者：王晓娜[1] 汪秀星[1] 赵茹茜[2] 薛春阳[1] 刘香[1] 陈杰[1,2] 

机构地区：[1]南京农业大学动物科技学院,南京210095 [2]南京农业大学动物医学院,农业部动物生理生化重点开放实验室,南京210095

出　　处：《遗传》2008年第11期1439-1447,共9页Hereditas（Beijing)

基　　金：国家自然科学基金重点项目(编号:30430420)资助~~

摘　　要：以皮特兰猪和二花脸猪为亲本建立的F2资源群体的52头猪进行2_h运输应激实验,将仔猪出生后第3、10和17 d的"Backtest"评分(BS3、BS10、BS17)以及运输后各个体的血浆应激和代谢相关激素水平Insulin、ACTH、Cortisol、T3和T4作为运输应激性状指标。利用22个微卫星标记,对这些应激指标进行相关性分析。结果表明:各位点等位基因数为3～8个,杂合度为0.4155～0.7432,多态信息含量为0.3651～0.8150。方差分析显示:SW1808(P<0.01)和SW0320(P<0.05)对ACTH有显著影响;SW1303对Insulin、SW1092对Cortisol、SW0320对T3、S0101对T4以及SW2446对BS3影响达到显著水平(P<0.05);SW2446(P<0.01)、SW1816(P<0.05)和SW0092(P<0.05)对BS10有显著影响;SW2108、SW1816和SW1023对BS17有显著影响(P<0.05)。结果表明这11个微卫星标记对实验中的应激性状指标存在显著影响。In the present study, we analyzed the effects of 22 microsatellite DNA loci on behavior responses and plasma concentration of hormones related to stress and metabolism following transport trial in pigs. These subjects were selected from the segregating F2 generation of a Pietrain （PIE） x Erhualian （EHL） cross, and the two swine strains differ in stress tolerance. A so-called Backtest Score （BS） at 3, 10 and 17 days after birth （BS3, BS10 and BS17） was implemented to assess the behavior responses for each piglet. Plasma concentrations of Insulin, ACTH, Cortisol, T3 and T4 in pigs were measured after the 2 hours＇ transport. One-way ANOVA was applied to analyze the relation between microsatellite polymorphisms and stress tolerance markers including BS and concentration of hormones. The results revealed that the allelic number was 3-8, heterozygosity was 0.4155-0.7432 and polymorphism information content was 0.3651-0.8150. SW1808 （P〈0.01） and SW0320 （P〈0.05） had significant effect on ACTH. The effect of SW1303 on Insulin, SW1092 on Cortisol, SW0320 on T3, S0101 on T4, and SW2446 on BS3 reached a significant level at P〈0.05, respectively. The effects of SW2446 （P〈0.01）, SW1816 （P〈0.05） and SW0092 （P〈0.05） on BS10 were significant, and the effects of SW2108, SW1816 and SW1023 on BS17 were also significant （P〈0.05）. Our study suggested that these 11 microsatellites in swine were closely associated with behavior responses and stress tolerance in response to transport.

关 键 词：猪 微卫星标记 运输应激 翻背测试 应激性状 相关分析 

分 类 号：S858.28[农业科学—临床兽医学]