大鳞副泥鳅3个Dmrt基因DM保守区的序列分析  

Sequence analysis of the DM domain of three Dmrt genes in loach, Paramisgurnus dabryanus

作　　者：徐玲花[1] 严镇钧[2] 曾庆韬[3] 

机构地区：[1]黄石理工学院化学与材料工程学院,湖北黄石435003 [2]湖北师范学院生命科学学院,湖北黄石435003 [3]湖北大学生命科学学院,武汉430062

出　　处：《遗传》2008年第11期1448-1452,共5页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30470970)资助~~

摘　　要：利用简并PCR克隆技术,扩增和克隆了大鳞副泥鳅(Paramisgurnus dabryanus)Dmrt基因的DM结构域,获得了3个具有不同DM序列的克隆。结果表明,在大鳞副泥鳅基因组中存在Dmrt基因家族的多个成员。同源性比较和系统进化分析显示不同进化地位脊椎动物的Dmrt基因存在高度的进化保守性。We amplified genomic DNA of the Paramisgurnus dabryanus using the DM degenerate primers and detected a band, approximately 150 bp. After cloned into pMD18-T vector and sequenced, three sequences showed high homology with the DM domain. They were named as PdDmrtl, PdDmrt3, and PdDmrt5. Based on similarities of amino acid sequences of the DM domain, Dmrt gene protein sequences from six vertebrates species were included in a phylogenetic tree. It confirmed the identity of the three PCR products. Our results reveal that Dmrt gene is highly conservative in phylogeny.

关 键 词：大鳞副泥鳅 DM结构域 DMRT基因 

分 类 号：S917.4[农业科学—水产科学]