靛酚蓝-分光光度法测定胃肠道内容物中氨态氮含量研究  

Determination of NH_4^+-N content in castrointestinal with indophenol blue spectrophotometric method

作　　者：蒲万霞[1,2] 魏云霞[2] 孟晓琴[1] 王玲[2] 陈炅然[2] 杨志强[2] 

机构地区：[1]甘肃农业大学动物医学院．甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所．甘肃兰州730050

出　　处：《甘肃农业大学学报》2008年第5期13-17,共5页Journal of Gansu Agricultural University

基　　金：中央级公益性科研院所基本科研业务费专项资金(BRF060303)

摘　　要：对靛酚蓝-分光光度法检测胃肠道内容物中氮含量的试验条件进行了筛选,结果表明:催化剂质量浓度25 mg/L,在640 nm的波长下,40℃水溶显色20 min为最佳检测试验条件;氨态氮标准曲线相关系数为0.999 1,线性范围为0~5.0μg,摩尔吸光系数ε640=1.26×103 L/(mol.cm),最低检出限为0.127μg;该方法与比色法的测定结果间无显著性差异,可用于胃肠道内容物中氨态氮含量的检测.The impact factors of determination of NH4^＋-N content in gastrointestinal contents were screened with indophenol blue spectrophotometrie method. The results showed that the optimum factors were reaction temperature at 40 ℃, reaction time for 20 rain, catalyst at 25 mg/L and determinate at 640 nm,respeetively. A linear work curve of NH4^＋-N with Rz = 0. 999 1 had been achieved,with 0-5.0μg of linearity extension and ε640 = 1.26 × 10^3 L/（mol · cm）. No significant difference in determination results was found between this method and colorimetry method. It was suggested that indophenol blue spectrophotometric method can be used as a method to determine NH4^＋-N content in gastrointestinal contents.

关 键 词：靛酚蓝-分光光度法 胃肠道内容物 氨氮 

分 类 号：Q5-33[生物学—生物化学]