实验用封闭群金黄地鼠mtDNA D-环核苷酸组成与变异分析  

Analysis of nucleotide content and variability of mtDNA D-loop sequence in laboratory closed colony of golden hamster

作　　者：贾晓蕊[1] 宁磊[2] 吴润[1] 景志忠[3] 王川[1] 赵春林[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]兰州生物制品研究所,甘肃兰州730046 [3]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《甘肃农业大学学报》2008年第5期29-32,共4页Journal of Gansu Agricultural University

摘　　要：对兰州、上海、北京和武汉4个封闭群共98只金黄地鼠线粒体DNA(mtDNA)D-环进行分析.结果表明:实验用封闭群金黄地鼠mtDNA D-环序列中,T、C、A、G碱基含量分别为30.9%2、5.2%、29.5%、14.4%,其中A+T为60.4%、G+C为39.6%,G+C含量明显低于A+T;发现的167个多态位点中,113个转换、52个颠换、2个位点既有转换也有颠换,分别占多态位点数的67.7%、31.1%1、.2%,没有插入/缺失同时出现的位点,表明我国实验用封闭群金黄地鼠个体间多态性丰富,群体间无明显变异.98 golden hamsters from four laboratory closed colonies of Lanzhou,Shanghai,Beijing and Wuhan were used to analyze the mitochondrial DNA （mtDNA） D-loop. The results showed that the percentage of T,C, A,G in the mtDNA D-loop sequences of the laboratory closed colony golden hamster were 30.9% ,25.2 % ,29.5% and 14.4 % respectively,in which A＋T was 60.4 % ,G＋C was 39.6 % ,and the content of A＋T was higher than G＋C. Total of 167 polymorphism sites were found,in which 113 transition,52 transversion and 2 of both transition and transversion,the percentage of these sites were 67.66 0%, 31.14% and 1.20 % respectively. However,there was no insertion/deletion. These indicated that Laboratory closed colony golden hamster in China has more rich of individual polymorphism and no-clear variation.

关 键 词：封闭群金黄地鼠 MTDNA D-环 遗传变异 

分 类 号：S865.142[农业科学—野生动物驯养]