藤本月季组织培养初报  

Preliminary report on tissue culture and rapid propagation of climbing rose

作　　者：杨永花 张美玲[2] 张新瑞[3] 廖伟彪[4] 

机构地区：[1]兰州市园林科学研究所,甘肃兰州730070 [2]甘肃农业大学理学院,甘肃兰州730070 [3]甘肃农业大学草业学院,甘肃兰州730070 [4]甘肃农业大学农学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2008年第5期63-66,共4页Journal of Gansu Agricultural University

基　　金：兰州市科技局科技攻关项目(编号:05-1-39)

摘　　要：以MS为基本培养基,附加不同浓度的6-BA、NAA、KT等植物生长调节剂,对藤本月季“莫扎特”品种的组培快繁技术进行了研究,同时在4-10月进行了试管苗移栽基质筛选试验.结果表明：适宜的诱导培养基为MS＋6-BA0.5 mg/L＋NAA0.05 mg/L,腋芽萌发率达220%,平均芽高为2.6 cm;增殖培养基以MS＋KT0.5 mg/L＋NAA0.5 mg/L效果最好,增殖系数达4.2,且芽生长健壮;最适宜的生根培养基为1/2MS＋IBA0.1 mg/L,生根率可达95%,且根粗壮发达.在移栽试验中,最佳的移栽基质为蛭石,且每年适宜在5、6月和9月进行试管苗移栽,其移栽成活率高达90%～95%.The effects of differ tablishment of subcultures and roo oncentrations of 6-BA, IAA, NAA, and KT were studied on the escultures of climbing rose（Rosa chinensis cv. Mozart）. The results showed that the suitable induction culture media was MS＋ 6-BA 0. 5 mg/L＋NAA 0. 05 rag/L, for the sprouting rate of axillary bud up to 220% and average growth height of bud was 2.6 cm. The optimum medium for multiplication was MS＋KT 0.5 mg/L＋NAA 0.1 mg/L,with the multiplication coefficient up to 4.2,and the buds grew strongly. 1/2MS＋IBA 0.1 mg/L was the optimum medium for rooting,with rooting percentage was 95%, the roots developed well. Transplanting survival rate was obviously high with vermiculite as media. The transplanting survival rate was up to 90%-95% in May,June and September.

关 键 词：藤本月季 组织培养 MS培养基 

分 类 号：S685.12[农业科学—观赏园艺]