温补散结方对体外诱导小鼠髓源性树突状细胞影响的实验研究  

The Experimental Research of Wenbusanjie Decoction on Dentritic Cell Derive from Mouce Marrow Cell in Vitro

作　　者：何欣[1] 邹彩亮[2] 王素红[2] 黄立中[3] 

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]湖南中医药大学,硕士生长沙410007 [3]湖南中医药大学中西医结合学院,长沙410208

出　　处：《中国中医急症》2008年第11期1570-1572,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(No.05JJ30027);湖南省教育厅重点资助项目(No.05A033)

摘　　要：目的观察温补散结方对体外诱导小鼠髓源性树突状细胞(DC)形态学及表面抗原表达的影响。方法无菌取小鼠骨髓细胞,置于含rmGM-CSF、rmlL-4及不同浓度中药培养液培养,以培养液中不含中药的组为空白对照组。于培养第24小时、第4、7日倒置显微镜观察各组培养的细胞形态,拍照记录。培养第8日,流式细胞仪分析各组培养细胞CD11c、CD80阳性率。结果形态学观察表明,高、低浓度的温补散结方均能够促进小鼠髓源性DC的分化成熟;温补散结方高、低浓度组CD11c阳性率、CD80阳性率、CD11c/CD80阳性率均较空白对照组高,差异均有显著性;中药高、低浓度组间CD11c阳性率、CD80阳性率、CD11c/CD80阳性率差异无显著性。结论温补散结方在体外能提高小鼠骨髓细胞诱导成DC的数量及共刺激分子表达,促进其分化成熟。Objective： To observe the effects of Wenbusanjie （WBSJ） Decoction on the cytomorphology and surface antigen presentation of dendritic cell （DC） derive from mouse marrow cell in vitro. Methods： The marrow cell were get from mouse, culture the cells in media with different concentration of WBSJ Decoction solution and recom- bined mouse granulocyte -macrophage colony- stimulating factor （rm GM -CSF）, recombined mouse interleukin -4 （rm IL - 4）. Observed the cytomorphology on 24h, 4, 7d by inverted microscope. Measure the positive ration of CD11c and CD80. Results： The cytomorphology observation showed WBSJ Decoction with high and low concentration could promote the mature of DC derive from mouse marrow cell. The ratio of CDt,c positive, CD80 positive, both CD11c and CD80 positive in the marrow cell cultured with high and low concentration. WBSJ Decoction were significantly higher than blank control group. There were no significant different between high and low concentration groups. Conclusion： WBSJ Decoction can improve the quantity and the presentation of eostimulating factor of DC derive from mouse marrow cell, promote the mature of DC in vitro.

关 键 词：温补散结方 树突状细胞 CD11C CD80 小鼠 

分 类 号：R285.5[医药卫生—中药学]