红鲌属3种鱼类线粒体细胞色素b基因片段序列分析  

Sequence Analysis of Mitochondrial Cyt b Gene Fragments of Three Species of Erythroculter

作　　者：冯晓宇[1] 谢楠[1] 李行先[1] 许宝青[1] 

机构地区：[1]浙江省杭州市农业科学研究院,杭州310024

出　　处：《西南大学学报（自然科学版）》2008年第10期79-83,共5页Journal of Southwest University(Natural Science Edition)

基　　金：浙江省杭州市科技计划资助项目(20052432B16)

摘　　要：测定了红鲌属的翘嘴红鲌、青梢红鲌和蒙古红鲌细胞色素b基因片段序列(1 091 bp),对其碱基组成变异情况及核苷酸序列差异进行了分析,并以红鳍鲌为外类群,用邻接(NJ)法和非加权算术平均对数(UPGMA)法构建系统关系树.结果表明:①4种鱼(包括外类群)Cyt b基因片段序列碱基平均差异为6.2%,变异位点184个,具有简约性信息位点141个,平均转换颠换比为7.4;②测定的红鲌属3种鱼形成一个单系群,其中翘嘴红鲌与青梢红鲌亲缘关系较近.Mitochondrial cytochrome b gene fragments of Erythroculter ilishaeformis, E. dabryi dabryi and E. mongolicus mongolicus were sequenced. The sequence divergence, base composition and base sub- stitution among these sequences were analyzed. Phylogenetic trees based on the sequences of the four species studied, including C. erythropterus as an outgroup, were constructed by neighbor-joining method （NJ） and unweighted pair group method with arithmetic mean method （UPGMA）, respectively. The results demonstrated that. （1） there were 184 variable sites and 141 parsim-info sites among cyt b of the four fishes, and the transition/transversion ratio was 7.4. （2） E. ilishaeformis, E. dabryi dabryi and E. mongolicus mongolicus formed a monophyletic group and E. ilishaeforrnis and E. dabryi dabryi had the closest phylogenetic relationship.

关 键 词：红鲌属(Erythroculter) 细胞色素b(Cytb) 序列分析 

分 类 号：S965.123[农业科学—水产养殖]