苯酚-硫酸法测定茉莉花多糖的研究  

Determination of Polysaccharide from Jasminum Sambac by Anthrone-sulfuric Acid Method

作　　者：董海丽[1] 李跃中[1] 

机构地区：[1]淮南联合大学化工系,安徽淮南232000

出　　处：《安徽农学通报》2008年第21期65-65,148,共2页Anhui Agricultural Science Bulletin

基　　金：安徽省教育厅一般项目(KJ2008B180)

摘　　要：为建立茉莉花多糖分析方法,采用苯酚-硫酸法、正交试验设计优选测定条件,结果表明,最佳提取条件为苯酚用量1.0ml、硫酸用量4ml、保温时间25min、保温温度80℃。该法测重复性好、操作简便,是一种适宜的茉莉花多糖分析方法。In order to establish a spectrophotometric method for the determination of the polysaccharides in Jasminum sambae, we taked phenol- sulfuric acid method and L9 （34） orthogonal design to optimum the determination conditions the results shown that the better condition was as follows： 1. 0mL 5 % solution of phenol, sulfuric acid 4. OraL, 80℃ water bath for 25min. The method is simple and well reproducible, it is suitable for the determination of the polysaccharides in Jasminum sambac.

关 键 词：茉莉花 多糖 苯酚-硫酸法 

分 类 号：Q539[生物学—生物化学]