二氧化锰颗粒对Hela细胞DNA损伤的尺度依赖性毒作用(英文)  

Size-Dependent Toxicity of Dioxide Manganese Particles on DNA Damage in Hela Cells

作　　者：毛彩霞[1] 杨光涛[1] 乔永康[1] 李岩[1] 袭著革[2] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室和遗传调控与整合生物学湖北省重点实验室,武汉4300792 [2]军事医学科学院卫生和环境医学研究所环境毒理学研究室,天津300050

出　　处：《生态毒理学报》2008年第5期438-442,共5页Asian Journal of Ecotoxicology

基　　金：the Grant of National High-Tech R&D Program (863 Program)(No. 2006AA03Z330); the Grants of China National Key Technology R&D Programs (No. 2006BAI19B05; No. 2006BAJ02A10)

摘　　要：为了观察二氧化锰颗粒物所致的尺度依赖性DNA损伤作用,将纳米尺度二氧化锰颗粒物(Nano-MnO2)和常规尺度二氧化锰颗粒物(Nor-MnO2)所致的DNA损伤进行了对比研究.将Hela细胞分别暴露于不同浓度(0、100、200、400μg·mL-1)的Nano-MnO2和Nor-MnO2中,染毒24h,采用彗星实验检测Hela细胞的DNA损伤水平.结果表明:与对照组相比,Nano-MnO2和Nor-MnO2均可使彗尾DNA百分比(TailDNA%)和尾矩(TailMoment)显著增加(p<0.01);而在同一浓度水平上,Nano-MnO2所致的DNA损伤则比Nor-MnO2所致的DNA损伤更为严重(p<0.01).结果提示:二氧化锰颗粒对Hela细胞DNA损伤具有尺度依赖性毒作用,纳米尺度比常规尺度二氧化锰颗粒毒作用更强烈.A comparison was made for the size-dependent DNA damage induced by both nano dioxide manganese particles（Nano-MnO2）and normal size dioxide manganese particles（Nor-MnO2）. Hela cells were exposed to different concentrations of both Nano-MnO2 and Nor-MnO2 （0, 100, 200, 400μg·mL^-1）, respectively. The DNA damage of Hela cells was measured by the comet assay after 24h MnO2-cultivated. Results showed that the Tail DNA% and Tail Moment were both significantly increased after exposed to both Nano-MnO2 and Nor-MnO2 when compared with the control（p〈0.01 for all）. At the same concentration level, Nano-MnO2 could induce more serious DNA damage than Nor-MnO2（p〈0.01 for all）. The results suggested that Nano-MnO2 should have more serious toxic effect regarding to DNA damage of the Hela cells than that of Nor-MnO2.

关 键 词：纳米尺度二氧化锰颗粒物 常规尺度二氧化锰颗粒物 DNA损伤 彗星实验 HELA细胞 

分 类 号：R994[医药卫生—毒理学]