通塞脉微丸化学成分的HPLC/MS研究  

Study on Compounds in Tongsaimai Pellets by HPLC-MS

作　　者：李伟东[1,2] 蔡宝昌[1,2] 丁选胜[3] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏省中药炮制重点实验室,江苏南京210046 [3]中国药科大学,江苏南京210009

出　　处：《中华中医药学刊》2008年第11期2355-2358,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家863重大研究计划项目(2003AA2Z3247);江苏省科技厅科技攻关重大项目(BK2001209)

摘　　要：目的:研究通塞脉微丸中的化学成分。方法:通过高效液相色谱可将三萜、黄酮及有机酸类等10余种化学成分较好的分离。根据紫外光谱可大致判断其化合物类型,由电喷雾质谱得到各成分的分子量,进而推测出其中主要成分的可能结构。结果:发现可能有绿原酸、甘草苷、夏拂托苷、甘草素-4’-芹糖葡萄糖苷、异甘草素葡萄糖芹糖苷、木犀草苷、安格洛苷C、异甘草苷、木犀草素、异甘草素、甘草糖苷B、甘草酸单铵盐、甘草酸等13个化合物。结论:通过对通塞脉微丸化学成分的解析,为阐明通塞脉微丸的药效物质基础提供有力的证据。Objective： To study compounds in Tongsaimai Pellets. Methods ： A few chemical constituents belong to triterpenes, flavones and phenolic acids are isolated commendably. Their type can be determined by ultraviolet spectrum, their molecular weight can be obtained by electrospray mass spectroscopy, and their imaginable structure can be deduced. Resuhs：13 compounds in Tongsaimai Pellets were identified by HPLC -MSThey are chlorogenic acid, liquiritin, schaftoside, ligquiritigenin - 4＇- apiosyl - glucoside, licuraslde, luteoloside, angoroside C, isoliqulritin, luteolln, isoliq uirltigenin, licorice- glucoside B, mono -ammonium glycyrrhetate and glcyrrhizic acid. Conclusion： To elucidate pharmacodynamic substantial basis by analyzing chemical constituents in Tongsaimai pellet.

关 键 词：通塞脉微丸 HPLC/MS 化学成分 

分 类 号：R284.1[医药卫生—中药学]