益气温阳护卫法对哮喘大鼠GATA3_mRNA表达的影响  

Effect of Yiqi Wenyang Huwei Method on Expression of GATA3 in Rat Asthma Model

作　　者：余建玮[1] 薛汉荣[1] 洪广祥[1] 邱小华 邹叶青 程光宇[1] 付向春[1] 杨玉萍[1] 兰智慧[1] 

机构地区：[1]江西中医学院呼吸病研究所,江西南昌330006 [2]江西省分子医学研究中心,江西南昌330006

出　　处：《中华中医药学刊》2008年第11期2377-2379,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30560188);江西省自然科学基金资助项目(0540112)

摘　　要：目的:探讨益气温阳护卫法对哮喘大鼠Th细胞异常分化相关分子基因表达影响。方法:50只SPF级雄性SD大鼠随机分为正常对照组、哮喘模型组、益气温阳护卫组、地塞米松组和卡介菌多糖核酸组,每组10只。采用TaqMan Realtime PCR测定各组外周血单个核细胞(PBMC)中GATA3_mRNA的表达变化。结果:哮喘模型组PBMC中GATA3_mRNA表达显著高于正常对照组(P<0.01),益气温阳护卫组、地塞米松组和卡介菌多糖核酸组PBMC中GATA3_mRNA的表达显著低于哮喘模型组(P<0.01),且与正常对照组PBMC中GATA3_mRNA的表达相当。结论:益气温阳护卫法调节Th1/Th2细胞亚群趋于平衡可能是通过抑制哮喘大鼠GATA3_mRNA的表达实现,从而达到防治哮喘的作用。Objective： Explore the effect of Yiqi Wenyang Huwei （YWH） method on gene expression of Th cell differentiation- related abnormal in a rat asthma model. Methods： Fifty SPF male SD rats were randomly divided into 5 groups： the normal control group, the asthma group, the YWH group, the dexamethasone group, the BCG - PSN group. The mRNA expression of GATA3 in the PBMC of all groups were detected hy TaqMan Real time PCR. Results ： The mRNA expression of GATA3 in PBMC of asthma group were significantly higher than that of control group（ P 〈 0.01 ）, the mRNA expression of GATA3 in PBMC of YWH group, the dexamethasone group and the BCG - PSN group was significantly lower than that of asthma group（ P 〈0.01 ） ,but corresponding with the normal control group. Conclusion： YWH method regulating Th1/Th2 cell subsets may be balanced by inhibiting the mRNA expression of asthmatic rats GATA3 achieve, so as to achieve the role of prevention and treatment of asthma.

关 键 词：益气温阳护卫法 哮喘 GATA3 TH1/TH2 

分 类 号：R289.5[医药卫生—方剂学]