脑囊虫病患者外周血单个核细胞体外诱生IL-2、IFN-γ及TNF-α活性的研究  

STUDIES ON THE ACTIVITY OF IL2、IFNγ AND TNFα INDUCED IN VITRO FROM PBMC OF PATIENTS WITH CEREBRAL CYSTICERCOSIS

作　　者：张唯哲[1,2] 徐之杰[1,2] 赵育莹[1,2] 王文余[1,2] 

机构地区：[1]哈尔滨医科大学寄生虫学教研室 [2]哈尔滨医科大学免疫学教研室

出　　处：《寄生虫与医学昆虫学报》1997年第4期207-210,共4页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：本文采用生物活性测定法检测了３１例脑囊虫病患者外周血单个核细胞（ＰＢＭＣ）体外诱生ＩＬ－２、ＩＦＮ－γ及ＴＮＦ－α活性，以３０例正常人作对照，探讨了脑囊虫病人细胞因子的变化及在免疫调节中的作用。结果显示：脑囊虫病患者ＰＢＭＣ体外诱生的ＩＬ－２、ＩＦＮ－γ水平明显降低，与正常人相比差异显著（Ｐ＜０．０５，Ｐ＜０．０１），而患者ＰＢＭＣ体外诱生的ＴＮＦ－α水平明显高于正常对照组（Ｐ＜０．０１）；提示脑囊虫病患者的ＣＤ＋４Ｔｈ１细胞呈低应答状态，脑囊虫病患者过量产生的ＴＮＦ－α可能是造成脑囊虫病的免疫病理损害原因之一。o investigate the levels of cytokines in peripheral blood mononuclear cells (PBMC) from patients with cerebral cysticercosis,We assayed IL2、IFNγ and TNFα production in PBMC　in vitro from 31 patients with cerebral cysticercosis and 30 healthy controls with bioassay methods.The results showed that IL2 and IFNγ production in culture supernatants from PBMC in patient group was significantly lower than that in control group,but TNFα production in culture supernatants from PBMC in patient group was significantly higher than that of control group.These results suggested that the responsiveness of CD+4Th1 is much suppressed in patients with cerebral cysticercosis as evidenced by decreased IL2 and IFNγ induced from PBMC,while a high level of TNFα induced in vitro from PBMC of patients with cerebral cysticercosis may be one of the reasons leading to immunopathological damages of patient.

关 键 词：脑囊虫病 外周血 单个核细胞 IFN-Γ TNF-α 

分 类 号：R532.330.3[医药卫生—内科学]