光照敏感型水稻红根突变体遗传分析及基因的分子定位  

Genetic Analysis and Molecular Mapping of Light-Sensitive Red-Root Mutant in Rice(Oryza sativa)

作　　者：张俊芝[1] 刘晓[1] 李超[1] 肖珂[1] 董彦君[1] 

机构地区：[1]上海师范大学生命与环境科学学院植物遗传与功能基因研究室,上海200234

出　　处：《中国水稻科学》2008年第6期578-582,共5页Chinese Journal of Rice Science

基　　金：上海市教育委员会资助项目(06ZZ21);上海市科学技术委员会资助项目(06PJ14074;075405117);国家948计划重大项目(2006-G1);国家航天育种工程资助项目(2006HT100013)

摘　　要：在自然光条件下水培籼稻品种Nankinkodo中,发现根为红色的自然突变体,命名为HG1。水培条件下该突变体在光照强度大于29μmol/(m2.s)的可见光下,根开始转红,在光照强度为180μmol/(m2.s)的可见光下,呈鲜红,具有明显的光照敏感性。遗传分析表明,该突变体光照敏感性的红根性状由1对显性基因控制,暂命名为Lsr。利用微卫星标记将Lsr基因定位在第4染色体上RM252与RM303之间,遗传距离分别为9.8c M和6.4c M,这为Lsr基因的精细定位和克隆奠定了基础。The light-sensitive red-root mutant, designated as HG1, was newly observed in an indica rice variety, Nankinkodo, when the roots were exposed to natural light under hydro-culture conditions. The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 /μmol/（m2 · s）, and turned dark-red at 180 μmol/（m2·s）, which suggested that the root color of the mutant was very sensitive to light. Furthermore, genetic analyses showed that the character of light-sensitive red-root in the HGl mutant was controlled by a single dominant gene, tentatively designated as Lsr. With the SSR markers, Lsr gene was mapped between RM252 and RM303 on chromosome 4 with the genetic distances of 9.8 cM and 6.4 cM, respectively. The results obtained from the study could be useful for fine mapping and cloning of Lsr gene in rice.

关 键 词：水稻 光照敏感性 红根突变体 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]