全反式维甲酸对血管平滑肌细胞增殖和转化生长因子-β mRNA表达的影响  

Effects of ATRA on DNA synthesis and expression of TGF β mRNA in vascular smooth muscle cells

作　　者：余晨光[1] 章鲁[1] 顾培坤[1] 魏丕敬[1] 金正均[1] 

机构地区：[1]上海第二医科大学神经生物学室

出　　处：《中国药理学通报》1997年第4期305-307,共3页Chinese Pharmacological Bulletin

基　　金：上海市高等学校科学技术发展基金

摘　　要：目的：研究全反式维甲酸对血管平滑肌细胞增殖，ＤＮＡ合成和转化生长因子－β（ＴＧＦ－β）基因表达的影响。方法：采用血管平滑肌细胞培养，细胞计数，３Ｈ－ＴｄＲ参入，斑点杂交和图像分析方法。结果：全反式维甲酸（Ａ－ＴＲＡ，０．０１，０．１，１μｍｏｌ·Ｌ－１）明显抑制胎牛血清（ＦＣＳ）促进的血管平滑肌细胞增殖和ＤＮＡ合成，其最大抑制率分别是２２．４％和８７．１％；全反式维甲酸明显抑制ＦＣＳ促进的血管平滑肌细胞ＴＧＦ－βｍＲＮＡ表达。结论：ＡＴＲＡ具有抗血管平滑肌细胞增殖作用，其机制与抑制ＴＧＦ－β基因表达有关。AIM: To study the effects of all trans retinoic acid (ATRA) on proliferation, DNA synthesis, and expression of TGF β mRNA in vascular smooth muscle cells(VSMC). METHODS: VSMC culture, cell count, 3H thymidine incorporation, dot blot and digital image processing were used. RESULTS: (1) ATRA(0 01, 0 1, 1 μmol·L -1 ) significantly inhibited FCS (200 ml·L -1 ) induced VSMC proliferation, DNA synthesis and their maxmal inhibitory rate at 1 μmol·L -1 were 22 4% and 87 1% respectively. (2) FCS (200 ml·L -1 ) increased expression of TGF β mRNA in VSMC; The effect was significantly inhibited by ATRA at 0 1 μmol·L -1 . CONCLUSIONS: ATRA has antiproliferation of VSMC and its mechnisms is related to inhibitting the expression of TGF β mRNA in VSMC.

关 键 词：全反式维甲酸 血管平滑肌细胞 TGF-Β mRNA 增殖 

分 类 号：R977.21[医药卫生—药品]