羊痘病毒双抗体夹心ELISA检测方法的建立  

Development of Double Antibody Sandwich ELISA for Detection of Capripoxvirus

作　　者：吴国华[1] 张强[1] 颜新敏[1] 李健[1] 朱海霞[1] 邵长春[1] 邬向东[2] 

机构地区：[1]中国农业科学院兰州兽医研究所／家畜疫病病原生物学国家重点实验室／农业部畜禽病毒学重点开放实验室,甘肃兰州730046 [2]江西农业大学动物科学技术学院,江西南昌330045

出　　处：《江西农业大学学报》2008年第5期860-864,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家重点基础研究发展计划(973)项目(2005CB523201);国家基础平台项目(2005DKA21205-3)

摘　　要：用纯化的山羊痘病毒分别免疫家兔和豚鼠,制备高效价的抗血清。经方阵滴定测定捕获抗体、检测抗体、酶结合物和标准抗原的稀释倍数。通过对阴性样品的检测,确定阴阳性的判定标准,初步建立羊痘病毒间接夹心ELISA诊断方法。用建立的ELISA方法对31份已知背景样品进行检测,总符合率为93.5%。经特异性试验、批内重复试验和对比试验,说明建立的夹心ELISA诊断方法具有良好的特异性、重复性和敏感性。对田间样品的检测表明,该ELISA诊断方法可应用于对羊痘的鉴别诊断。The antisera against purified goatpox virus were obtained from rabbits and guinea pigs. The optimal dilution of antisera and antigen were determined by titration test. After the establishment of double antibody sandwich ELISA, 31 samples were detected. The results showed that the coincidence rate was 93.5%. It was proved that the method had good specificity , repeatability and sensitivity. Detection of field samples suggested that the established ELISA could be used to detect capripoxvirus.

关 键 词：羊痘病毒 夹心ELISA 诊断 

分 类 号：S852.654[农业科学—基础兽医学]