海马微注射秋水仙碱对小鼠学习记忆的影响及CGP36742的保护作用  

Effect of hippocampal microinfusion of colchicine on learning and memory, and protective function of CGP36742 in mice

作　　者：俞在芳[1] 胡本荣[1] 程冠军[1] 

机构地区：[1]中山医科大学药理学教研室

出　　处：《中国药理学与毒理学杂志》1997年第4期246-249,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：小鼠每侧海马微注射秋水仙碱（Ｃｏｌ）２．８μｇ１４ｄ后，用跳台法和避暗法测验显示错误数显著增加，潜伏期显著缩短，学习记忆出现障碍，同时大脑皮层及海马ｃＡＭＰ含量显著下降；γ－氨基丁酸Ｂ受体拮抗剂３－氨丙基－丁基磷酸（ＣＧＰ３６７４２）（５０ｍｇ·ｋｇ－１·ｄ－１，ｉｐ）于海马微注射Ｃｏｌ后治疗１４ｄ可显著改善Ｃｏｌ所致的学习记忆障碍，并升高ｃＡＭＰ含量．海马微注射生理盐水后给予ＣＧＰ３６７４２５０ｍｇ·ｋｇ－１·ｄ－１ｉｐ１４ｄ，小鼠跳台和避暗实验错误数明显减少．说明学习记忆功能增强，同时大脑皮层，海马和小脑ｃＡＭＰ含量升高．结果提示脑内ｃＡＭＰ含量升高对学习记忆是促进因素，ＧＡＢＡＢ受体拮抗剂可望成为新型的抗老年性痴呆药物．Mice, infused with 2.8 μg of colchicine in 0.5 μL of saline into each hippocampus, have been shown a significant increase in the error numbers and a decrease in the latency in step down and step through tests fourteen days after the infusion. At the same time there was also a significant decrease in cAMP levels in the cortex and hippocampus. CGP36742(3 aminopropyl n butyl phosphinic acid), a GABA B receptor antagonist, at the dosage of 50 mg·kg 1 ip daily for 14 d, could attenuate the impairment of learning and memory and the decrease in cAMP levels in the mice treated by colchicine. Being used for 14 d after infused with saline in each hippocampus, CGP36742 could also improve learning and memory function and elevate cAMP levels in the cortex, hippocampus and cerebellum. The results suggest that the elevation of cAMP levels in the brain could be beneficial to learning and memory functions and GABA B receptor antagonist might be a novel type of drug to treat Alzheimer′s disease.

关 键 词：秋水仙碱 学习 记忆 GABAB 受体拮抗剂 

分 类 号：R971[医药卫生—药品]