海南岛立克次体病血清学和病原学调查  

SEROLOGICAL AND ETIOLOGICAL STUDIES ON UNDEFINED RICKETTSIOSIS ON HAINAN ISLAND

作　　者：林碧瑚[1] 孙晓娟[1] 詹志农[1] 林英姿[1] 李文广[1] 

机构地区：[1]海南医学院微生物学教研室

出　　处：《海南医学院学报》1997年第3期97-102,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金

摘　　要：作者从海南岛的东、南、西、北、中五个地区收集一般人群血清５６１份，不明热患者血液标本２０份，应用恙虫病、、斑疹伤寒、斑点热三种立克次体抗原片对血清标本同时进行ｍＩＦＡ测定。发现ＩｇＧ抗体阳性率分别为１３．５５％，１８．７７％，３０．８３％。ＩｇＧ滴度≥１∶８０的血清中，ＩｇＭ抗体也≥１∶８０者，斑疹伤寒为３２．０８％，斑点热为４６．３０％。２０份患者血液标本应用Ｌ９２９细胞进行立克次体分离培养，共分出１２株立克次体。用ｍＩＦＡ和ＰＣＲ初步鉴定出７株斑点热群立克次体。首次从血清学、病原学、遗传学上证实海南岛斑点热群立克次体病的存在。The paper reports the results of the serosurvey for antibodies to rickettsiae and rickettsial isolation,which confirmed the existence of SFG rickettsiosis on Hainan Island for the first time.Serum from 561 persons and blood samples from 20 patients with undiagnosed fevers were collected from all over the Island for the study and the results of the serosurvey for antibodies to rickettsiae with mIFA showed positive rates of antibody IgG to R.tsutsugamushi,R.partrii E,R.siberica were 13.55%,18.77%,and 30.83% respectively.In the positive sera with high titer IgG antibody(≥1∶80),the positive rates of antibody IgM with high titer(≥1:80) were 46.30%(25/34) in reaction to R.siberica and 32.08%(17/53) in reaction to R.partrii E.Isolation of rickettsiae from the 20 blood samples of FUO patients was carried out with cell culture techniques and 12 strains of rickettsia were isolated in L 929 monolayer cells.Identification of pathogens was performed with mIFA with specific antiserum and PCR techniques and the results indicated 7 strains of SFG rickettsia.

关 键 词：海南岛 立克次体病 血清学 病原学 

分 类 号：R513[医药卫生—内科学]