产生几丁质酶的食线虫真菌绿粘帚霉Gliocladium virens CFCC80915对南方根结线虫卵孵化的影响  

Effect of chitinase-producing nematophagous fungus Gliocladium virens CFCC80915 on egg hatching of Meloidogyne incognita

作　　者：于鹏飞[1] 武侠[1] 张成敏[1] 赵洪海[1] 才秀华[1] 

机构地区：[1]青岛农业大学植物保护学院,青岛266109

出　　处：《植物病理学报》2008年第5期496-500,共5页Acta Phytopathologica Sinica

基　　金：青岛市科技局课题(04-3-NN-01)

摘　　要：根结线虫卵壳主要由几丁质和蛋白质构成。寄生根结线虫卵或产毒真菌产生几丁质酶特性是评价食线虫真菌生物防治潜力的重要生化指标之一。利用还原糖法和pNP法分别测定绿粘帚霉(Gliocladium virens)的几丁质酶的内切酶和外切酶活性。第14d内切酶活性达到最高值,其酶活为53.1μmol/h mL;外切酶活性第26d达到高峰,其酶活为0.432μmol/h mL。利用活性染色电泳测其几丁质酶的分子量分别为75.8kDa、42.8kDa和39.6kDa。表明筛选到的绿粘帚霉CFCC80915菌株具有较高产生几丁质酶的活性。绿粘帚霉12d的培养滤液对根结线虫卵孵化7d后的抑制率达到92.9%。显微观察线虫卵壳变形和破坏情况的结果表明,绿粘帚霉CFCC80915产生的几丁质酶可以引起根结线虫卵壳的裂解,抑制根结线虫卵的孵化。The eggshells of Meloidogyne spp.are mainly composed of chitin and protein.The chitinase acti-vity of fungi which parasitize eggs or produce toxin for Meloidogyne spp.is one of the most important bioche-mical characters.It is used for evaluating the biocontrol potential of nematophagous fungi.The endochitinase and exodchitinase of Gliocladium virens were tested with NAG and pNP.The maximum activity of endochitinase was 53.1 μmol/h mL at the 14 d and the maximum activity of exodchitinase was 0.432 μmol/h mL at the 26 d.The molecular weights of chitinase were estimated as 75.8 kDa,42.8 kDa and 39.6 kDa.The results showed that G.virens CFCC80915 had high chitinase activity.After 7 days of incubation with the chitinase producing G.virens CFCC80915,inhibitory rate of eggs hatching achieved 92.9%.Microscopic observations demonstrated that the eggshell of Meloidogyne spp.was deformed and destroyed.The reseach indicated that chitinase produced by G.virens CFCC80915 caused the lysis of Meloidogyne spp.eggshells and resulted in the inhibition of egg hatching in vitro.

关 键 词：绿粘帚霉 几丁质酶 几丁质外切酶 根结线虫 卵孵化 

分 类 号：S432.45[农业科学—植物病理学]