大豆茎溃疡病菌TaqMan MGB探针实时荧光PCR检测  

Real-time fluorescent PCR detection of Diaporthe phaseolorum var. caulivora and D. phaseolorum var. meridionalis by using TaqMan MGB probe

作　　者：王颖[1] 陈枝楠[1] 赵巍巍[2] 程颖慧[1] 章桂明[1] 

机构地区：[1]深圳出入境检验检疫局,深圳518010 [2]湖南农业大学,长沙410128

出　　处：《植物病理学报》2008年第5期532-535,共4页Acta Phytopathologica Sinica

基　　金：国家质量监督检验检疫总局课题资助项目(2003IK071)

摘　　要：The technique of TaqMan MGB real-time fluorescent PCR was established to detect Diaporthe phaseolorum var.caulivora(DPC) and D. phaseolorum var.meridionalis (DPM). The primers and TaqMan MGB probes were designed based on the ITS of DPC, DPM, D. phaseolorum var. sojae and Phomopsis longicolla. A series of genomic DNA dilution were used to detect sensitivity of the technique, the results showed that the limits of detection for DPM and DPC were 7 fg/μL and 6 fg/μL DNA respectively.The technique of TaqMan MGB real-time fluorescent PCR was established to detect Diaporthe phaseolorum var.caulivora（DPC） and D. phaseolorum var.meridionalis （DPM）. The primers and TaqMan MGB probes were designed based on the ITS of DPC, DPM, D. phaseolorum var. sojae and Phomopsis longicolla. A series of genomic DNA dilution were used to detect sensitivity of the technique, the results showed that the limits of detection for DPM and DPC were 7 fg/μL and 6 fg/μL DNA respectively.

关 键 词：大豆生产 实时荧光PCR检测 溃疡病菌 TAQMAN 茎 毁灭性病害 探针 潜在威胁 

分 类 号：S565.1[农业科学—作物学] S435.4