反相高效液相色谱法测定益气降糖胶囊中葛根素的含量  

Content analysis of puerarin in Yiqijiangtang capsules by HPLC

作　　者：陆炜琨[1] 曾芳[1] 李媛[1] 

机构地区：[1]中山大学附属第一医院黄埔院区药剂科,广东广州510700

出　　处：《广东药学院学报》2008年第5期475-477,共3页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的建立益气降糖胶囊中葛根素的含量测定方法。方法采用高效液相色谱法,色谱柱为C18色谱柱(5μm,4.6mm×200mm);甲醇-0.5%醋酸(体积比19∶81)为流动相;流速1.000mL/min,检测波长250nm,柱温35℃。结果葛根素的进样量在0.0535～0.535μg范围内与峰面积呈良好的线性关系,r=0.9998;平均回收率为96.32%,RSD为0.63%。结论本方法简单、可靠、专属性强,可以作为控制益气降糖胶囊质量的方法。Objective To establish an HPLC method for analyzing the content of puerarin in Yiqijiangtang capsule. Methods Samples were analyzed on an LUNA ODS C18 column（5 m,4.6 mm 200 mm）by using methanol -0.5% HAc （19：81 ） as mobile phase. The flow rate was 1. 0 mL/min and the detecting wavelength 250 nm. Results The linear range of puerarin was 0. 0535 -0. 535 μg（r =0. 9998）. The average recovery was 96.32% （RSD = 0.63% ）. Conclusion The method is reliable, accurate and specific. It can be used for the quality control of Yioiiiangtang caosule.

关 键 词：益气降糖胶囊 葛根素 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]