RP-HPLC法测定盐酸洛美沙星胶囊的含量及有关物质  

Determination of Lomefloxacin Hydrochloride and its Related Substances in Lomefloxacin Hydrochloride Capsuless by RP-HPLC

作　　者：杜蓉[1] 刘辉[1] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070

出　　处：《中国药师》2008年第11期1337-1338,共2页China Pharmacist

摘　　要：目的:采用高效液相色谱法测定盐酸洛美沙星胶囊中盐酸洛美沙星的含量及其有关物质。方法:用Lichospher ODS- C_(18)柱(250 mm×4.6 mm,5μm);以0.05 mol·L^(-1)枸橼酸溶液(用三乙胺调节pH至4.0)-乙腈(87:13)为流动相;检测波长287 nm。结果:盐酸洛美沙星的线性范围为20～200μg·ml^(-1)(r=0.9995),平均回收率为99.7%(n=9)。结论:方法重复性好,准确度高,适于盐酸洛美沙星胶囊的质量控制。Objective： To establish a RP-HPLC method for determination the content of lomefloxaein hydrochloride and its related substances in lomefloxacin hydrochloride capsules. Method： the lichospher ODS-C18 column（250 mm×4.6 mm, 5 μm） was used the 0.05 mol.L^-1 citric acid solution-acetonitrile （87： 13） as mobile phase adjust pH to 4.0 with triethylamine） ;the detection wavelength was 287 nm. Result ： the linear range of tomefloxacin hydroxide was 20 - 200 μg· ml^ -1 （ r = 0. 999 5 ） ; the mean recovery was 99.7% with RSD 0. 3% （n = 3）. Conclusion： The method is sensitive, accurate and rapid. It is suitable for quality control of lomefloxacin hydrochloride capsules.

关 键 词：高效液相色谱法 盐酸洛关沙星胶囊 含量测定 有关物质 

分 类 号：R927.2[医药卫生—药学]