沙蚕激酶全基因在大肠杆菌中表达及溶栓作用检测  

THE EXPRESSION OF NEREIS KINASE AND ITS THROMBOLYTIC ROLE

作　　者：陆国辉[1] 赵百慧 李泰东[1] 张贵芝[1] 王斌 闫志勇 金毅[1] 

机构地区：[1]青岛大学医学院生理学教研室,山东青岛266021 [2]青岛市医药生物技术重点实验室

出　　处：《青岛大学医学院学报》2008年第6期495-497,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家"973"计划重大基础研究前期研究专项课题(2004CCA02400)

摘　　要：目的使沙蚕激酶全基因在大肠杆菌中高效表达获得沙蚕激酶,并检测其溶栓活性。方法通过BamHⅠ和EcoRⅠ双酶切将沙蚕激酶全基因克隆进载体pGEX4T-2,诱导表达后SDS-PAGE证实该基因正常表达。构建大鼠颈动-静脉旁路血栓模型,进行目的蛋白溶栓作用检测。结果沙蚕激酶使血栓湿质量减小,优球蛋白溶解时间(ELT)缩短(F=19.112、20.390,P<0.05),但对纤维蛋白原(FIB)影响不明显(F=1.333,P>0.05)。结论沙蚕激酶基因表达的目的蛋白有抑制血栓形成、激活纤溶活性的作用。Objective To obtain nereis kinase by effective expression of nereis kinase whole-gene in E. coil, and detect its thrombolytic activity. Methods Nereis kinase gene whole-gene was cloned into vector pGEX4T-2 by double-enzyme cut with BamH I ＆ EcoR I , the gene was confirmed to be normal. A rat carotid artery-vein-bypass thrombus model was established to determine the thrombolytic effect of the interest protein. Results The wet weight of thrombus decreased and ELT shortened （F= 19. 112, 20. 390;P〈0.05）, but no significant effect on FIB by nereis kinase was observed （F= 1. 333, P〉0.05）. Conclusion The interest protein expressed by nereis kinase gene has the effects on inhibiting thrombopoiesis and activating thrombolytic activity.

关 键 词：沙蚕激酶 基因表达 纤维蛋白溶解 大鼠 

分 类 号：R91[医药卫生—药学]