稻瘟菌粗毒素诱导水稻过程中CAT,PPO活性和MDA含量的变化  

Changes of CAT and PPO Activities and MDA Content of Rice during Induction by Rice Blast Crude Toxin

作　　者：叶漪[1] 范静华[1] 孟艳妮[1] 果志华[1] 陈建斌[1] 

机构地区：[1]云南农业大学植物病理重点实验室,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2008年第6期775-780,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金："973"前期专项(2003CCA02000)

摘　　要：利用不同浓度稻瘟菌粗毒素处理水稻幼苗，研究在不同浓度处理下稻瘟菌粗毒素对水稻种子萌发、水稻叶片中过氧化氢酶（CAT）、多酚氧化酶（PPO）活性、有害物质丙二醛（MDA）浓度变化的影响。结果表明，在Fries培养液中培养的粗毒素对水稻种子萌发，种子胚根、胚芽都有明显的抑制作用。经0～96h的动态检测，CAT活性比对照平均提高了17．5％，PPO活性比对照平均提高了13．1％，MDA的含量比对照平均降低了15．9％。说明经稻瘟菌粗毒素处理过的水稻能够诱导提高相关酶CAT，PPO的活性，减少有害物质MDA的含量。The seedlings of rice （ Oryzae satira） were treated with different concentrations of rice blast crude toxin, extracted from Pyricularia oryzae, to study the effect of rice blast crude toxin on the seed germination, the activities of catalase （CAT） , poly-phonoloxidase （PPO） and the content of malondialdehyde （MDA） in rice leaves. The results showed that the toxin significantly inhibited the germination rate, shoot and root elongation. During 0 - 96 h inspection, compared with the control, the activities of CAT and PPO increased 17.5% and 13. 1% , respectively, but the content of MDA decreased 15.9%. It was indicated that low concentration of filtration of rice race cultivation itself could be used as an inductor to enhance the CAT and PPO activities and reduce the MDA content.

关 键 词：水稻 粗毒素 多酚氧化酶 过氧化氢酶 丙二醛 诱导 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]