Rab27A在4种不同人乳癌细胞株中的表达  

EXPRESSIONS OF Rab27A IN FOUR KINDS OF HUMAN BREAST CANCER CELLS

作　　者：徐鹏[1] 刘松岭[2] 孙国锋[2] 曹明智[1] 马学真[3] 王劲松[4] 李文凤[1] 

机构地区：[1]青岛大学医学院附属医院乳腺病治疗中心,山东青岛266021 [2]青岛市肿瘤医院外一科 [3]青岛市肿瘤医院肿瘤内科 [4]复旦大学肿瘤医院乳腺外科

出　　处：《齐鲁医学杂志》2008年第6期492-493,495,共3页Medical Journal of Qilu

摘　　要：目的检测Rab27A在4种人乳癌细胞中的定位、表达情况,初步研究Rab27A表达对乳癌细胞生物学特性的影响。方法采用RT-PCR技术,检测Rab27A mRNA在4种人乳癌细胞MCF-7、MDA-MB-231、MDA-MB-435和MDA-MB-435HM中的表达并进行半定量分析。结果Rab27A mRNA在人乳癌细胞MDA-MB-231、MDA-MB-435及MDA-MB-435HM中的表达水平分别是MCF-7中的2.1、3.4和6.9倍,差异有显著意义（F=17.74～136.23,P〈0.05、0.01）;Rab27A蛋白在人乳癌细胞MDA-MB-231、MDA-MB-435及MDA-MB-435HM中的表达分别是MCF-7中的2.8、4.9和9.2倍,差异有显著性（F=37.74～154.29,P〈0.05、0.01）。Rab27A蛋白弥散性分布于MCF-7、MDA-MB-231和MDA-MB-435细胞浆中,MDA-MB-231和MDA-MB-435中又可见Rab27A蛋白在核周凝聚。结论Rab27A表达可能与乳癌细胞侵袭转移能力有关。Objective To investigate the expression of Rab27A in four kinds of human breast cancer cells and its effect on cytobiologic characteristics of the cells. Methods The mRNA of Rab27A was detected and semiquantitative analysis done by RT-PCR in four kinds of breast cancer cells, i.e. MCF-7, MDA-MB-231, MDA MB-435,aud MDA-MD435HM. Results The levels of Rah27A mRNA in MDA MB-231, MDA-MB-435 and MDA MB-435HM were 2.1, 3.4 and 6.9 times, respectively, that in MCF-7 （F= 17. 74--136.23; P〈0. 05,0. 01）. Rab27A protein expression in MDA-MB-231, MDA-MB-435 and MDA-MB- 435HM was 2. 8, 4.9 and 9.2 times that inMCFT, respectively （F=37.74--154.29;P〈0.05,0.01）. Rab27A was found to be targeted to diffuse in cytoplasm in three cancer cell lines and particularly concentrated in perinuclear in MDA-MB 231 and MDA-MB-435 cells. Conclusion The expression of Rab27A is probably related to cancer cell potential of invasion and metastasis.

关 键 词：乳腺肿瘤 Rab27A 肿瘤转移 

分 类 号：R73-3[医药卫生—肿瘤]