云南省两例输入性脊髓灰质炎Ⅰ型野病毒病例的分子病毒学检测  

IDENTIFICATION OF TWO WILD TYPE I POLIOVIRUSESISOLATED IN YUNNAN PROVINCE BY MOLECULAR VIROLOGY

作　　者：李杰[1] 郑红[1] 徐闻 方勇[1] 朱宏[1] 张礼壁[1] 

机构地区：[1]中国预防医学科学院国家脊髓灰质炎实验室,云南省卫生防疫站脊髓所质炎实验室

出　　处：《中国计划免疫》1997年第2期58-61,共4页Chinese Journal of Vaccines and Immunization

摘　　要：１９９５年末和１９９６年初，各有１名缅甸儿童到我国云南省就医，临床诊断为脊髓灰质炎（脊所）。从患者的粪便体本中各分离到１株病毒，经血清学定型均为脊灰１型。经ＰＣＲ－ＲＦＬＰ法检测，两株病毒的酶切图谱完全一致，但与Ｓｄｂｉｎ１有明显差异，判断为野毒株。对相同片段的核苷酸序列测定结果肯定了这一判断。其核苷酸序列经ＰＨＹＬＩＰ软件分析，显示其基因型与我国境内曾经流行过的野毒株有明显差异，说明这两株野毒株是由缅甸传人中国的“输入野毒株”。Two patients with Myammar nationality were diagnosed as poliomyelitis in a hospital of Yunnan Province. Type 1 polioviruses were isolated from their stool samples. The intratypic identification of the two isolates was carried out by PCR - RFLP method. The re- sults showed the two isolates had identical profiles, but very different from those of Sabin I, they were wild polioviruses . The phylogenetic character of the two isolates when com- pared with the other epidemic strains circulated in China before by PHYLIP software, showed large differences among them as seen in the dendrogram. This proves that the two isolates were'imported wild strains' from Myarumar.

关 键 词：脊髓灰质炎 Ⅰ型病毒 野毒标 基因型 

分 类 号：R512.4[医药卫生—内科学] R373.22[医药卫生—临床医学]