广东省1985～1993年脊髓灰质炎流行野毒株的基因分析  

GENETIC ANALYSIS OF EPIDEMIC WILD POLIOVIRUSES ISOLATED IN GUANGDONG 1985-1993

作　　者：柯昌文[1,2] 吴承民[1,2] 李杰 张礼璧[1,2] 郑焕英 林伟生[1,2] 罗耀星[1,2] 林协勤 林永杰[1,2] 陈树本 

机构地区：[1]广东省卫生防疫站 [2]中国预防医学科学院国家脊灰实验室

出　　处：《中国计划免疫》1997年第6期268-272,共5页Chinese Journal of Vaccines and Immunization

摘　　要：广东省是脊髓灰质炎（脊灰）高发区，１９８５～１９９３年各年都有流行，历年流行的脊灰野毒株均为Ｉ型病毒。在历年来分离的流行病毒株中，选择不同时期的Ｉ型脊灰病毒２２株做ＰＣＲ－ＲＦＬＰ分析，发现广东省脊灰Ｉ型流行株共有９种电泳图谱类型，显示了在基因水平上的多样性。根据ＰＣＲ－ＲＦＬＰ分析结果又选择了其中９株代表株，对其ＶＰ１编码区３００个核苷酸进行序列分析，结果显示各年的流行株间序列有差异。此结果表明，广东省脊灰流行与多种基因型脊灰病毒的存在有关，但口服脊灰疫苗（ＯＰＶ）形成的免疫屏障可阻断脊灰野病毒的传播。 From 1985 to 1993,poliomyelitis outbreak occurred annually in Guangdong Province All the epidemic strains were wild type I polioviruses, 22 epidemic strains were analyzed by PCR-RFLP and 9 types of electrophoresis profile were shown 300 nucleotides in VP1 coding region (nucleotides 2,480-2,779) of 9 representative epidemic strains were compared with each other by sequence analysis It showed clearly that the genotypes of polioviruses isolated from each outbreak were differentThis study indicates that there will bean outbreak when the poliovirus with a new genotype circulated, but the high OPV covergage rate is able to prevent the spread of wild type poliovirus

关 键 词：脊髓灰质炎 病毒 流行株 序列分析 基因型 

分 类 号：R512.4[医药卫生—内科学] R373.22[医药卫生—临床医学]