RP-HPLC法同时测定鸡冠花中槲皮素、木犀草素和山柰酚的含量  

Simultaneous determination of quercetin,luteolin and kaempferol in Celosia cristata L. By RP-HPLC

作　　者：王锦军[1] 张秀梅 黄兆文[1] 

机构地区：[1]荆楚理工学院药学院,湖北荆门448000 [2]荆门市第二人民医院,湖北荆门448000

出　　处：《西北药学杂志》2008年第6期354-356,共3页Northwest Pharmaceutical Journal

摘　　要：目的建立鸡冠花中槲皮素、木犀草素和山柰酚的含量测定方法。方法反相高效液相色谱法,色谱柱ZORBAXSB-C18(150mm×4.6mm,5μm);检测波长360nm;甲醇-0.2%磷酸(45∶55)为流动相;柱温30℃;流速1.0mL·min-1。结果槲皮素、木犀草素和山柰酚的回归曲线分别为:Y=1504.412X+9.9756,Y=1991.745X+8.6051,Y=567.591X+2.5397,它们分别在5.5×10-2～19.3×10-2mg·L-1,4.6×10-2～16.1×10-2mg·L-1,12.6×10-2～43.9×10-2mg·L-1范围内线性关系良好,相关系数r=0.99992～0.99998,加样回收率分别为94.68%,91.03%和103.08%,RSD分别为0.35%,1.01%和0.55%。样品分别含槲皮素、木犀草素和山柰酚0.176,0.262和0.105mg.g-1。结论方法简便可行,重复性好,数据及结果可靠。Objective To develop an RP-HPLC for determination of three flavonoids（quercetin, kaempferol and luteolin） in Celosia cristata L. Method The H PLC column was ZORBAX SB-C18 （150 mm× 4.6 mm, 5 μm）, temperature was 30 ℃ ;detection wavelength was 360 nm ;mobile phase was menthol-0. 2%phosphonic acid （45 ： 55） ,flow rate was 1.0 mL · min^-1. Results Quercetin, luteolin and kaempferol regression equations were Y = 1 504. 412X ＋ 9. 975 6 ,Y = 1 991. 745X ＋ 8. 6051 and Y = 567. 591X ＋ 2.539 7 ,respectively ;Linear ranges were 5.5×10^-2-19.3×10^-2 mg· L^-1,4.6×10^-2-16.1×10^-2 mg· L^-1 and 12.6×10^-2- 43.9×10^-2 mg · L^-1 respectively ; corelation coefficents were between 0. 999 92 and 0. 999 98; recoveries were 94.68% ,91.03% and 103.08%, respectively ; RSD were 0. 35%, 1. 01% and 0. 55%, respectively. Sample contents of quercetin, luteolin and kaempferol were 0. 176,0. 262 and 0. 105 mg · g^-1 , respectively. Conclusions This method was convenient and effective, and suitable for the determination of quercetin,kaempferol and luteolin in Celosia cristata L.

关 键 词：鸡冠花 槲皮素 木犀草素 山柰酚 含量测定 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]