化学去细胞同种异体神经复合缓释神经生长因子修复周围神经缺损  

EFFECT OF CONTROLLED RELEASE NERVE GROWTH FACTOR ON REPAIRING PERIPHERAL NERVE DEFECT BY ACELLULAR NERVE GRAFT

作　　者：于海龙[1] 彭江[1] 孙华燕[2] 徐风华[2] 张莉[1] 赵斌[1] 眭翔[1] 许文静[1] 卢世璧[1] 

机构地区：[1]解放军总医院全军骨科研究所,北京100853 [2]解放军总医院制剂室,北京100853

出　　处：《中国修复重建外科杂志》2008年第11期1373-1377,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(30571875);北京市自然科学基金重点资助项目(03G502)~~

摘　　要：目的探讨化学去细胞异体神经复合缓释神经生长因子(nerve growth factor,NGF)修复周围神经缺损的效果。方法采用药物微球技术制备NGF微球,与生物纤维蛋白胶混合形成NGF复合缓释制剂。取健康雄性SD大鼠20只,体重280～300g,制备化学去细胞异体神经。取健康雄性Wistar大鼠52只,体重250～300g,制备大鼠左侧坐骨神经10mm缺损模型。根据修复缺损材料不同,随机分成4组(n=13):自体神经移植组(A组)、化学去细胞异体神经移植加NGF复合缓释制剂组(B组)、化学去细胞异体神经移植组(C组)和化学去细胞同种异体神经移植和纤维蛋白胶组(D组)。右侧不作处理作为空白对照组。术后行大体观察;术后2周测量神经轴突生长距离;术后16周行电生理检测,计算术侧坐骨神经运动传导速度恢复率、术侧小腿三头肌收缩力恢复率及湿重恢复率,并行移植神经吻合中段HE和半薄切片甲苯胺蓝染色观察。结果所有动物均存活至实验完成,切口愈合良好。术后2周A组神经再生距离较其余3组长(P<0.05),B组优于C、D组(P<0.05),C、D组间比较差异无统计学意义(P>0.05)。术后16周,A、B、C、D组术侧坐骨神经运动传导速度恢复率分别为73.37%±7.82%、70.39%±8.45%、53.51%±6.31%、55.28%±5.37%;A、B组与C、D组比较,差异有统计学意义(P<0.05)。A、B、C、D组术侧小腿三头肌收缩力恢复率分别为85.33%±5.59%、69.79%±5.31%、64.46%±8.49%、63.35%±6.40%;A组与B、C、D组比较差异有统计学意义(P<0.05),B、C、D组间比较差异无统计学意义(P>0.05)。A、B、C、D组术侧小腿三头肌湿重恢复率分别为62.54%±8.25%、53.73%±4.56%、46.37%±5.68%、45.78%±7.14%;A组与B、C、D组比较差异有统计学意义(P<0.05),B组与C、D组比较差异有统计学意义(P<0.05),C、D组间比较差异无统计学意义(P>0.05)。组织学图像分析示B组有髓神经纤维数量、轴突直径及髓鞘厚度均优于C、D组(P<0.05),B�Objective To explore the effect of controlled release of nerve growth factor （NGF） on peripheral nerve defect repaire by acellular nerve graft. Methods The microspheres of NGF were prepared with drug microsphere technology and fixed with the fibrin glue to make the complicated controlled release NGF. Twenty healthy male SD rats weighing 280-300 g were adopted to prepare acellular xenogenous nerve, 52 male Wistar rats weighing 250-300 g were adopted to prepare the 10 mm defect model of left sciatic nerve, and thereafter were randomly divided into 4 groups： autograft group（group A）, acellular nerve allograft combined with the double controlled release NGF （group B）, acellular nerve allograft （group C） and acellular nerve allograft combined with fibrin glue （group D）. Without any operation, the right sciatic nerve was regarded as control group. General observation was conducted after operation. The nerve axon regeneration length was measured 2 weeks after operation. The effects of peripheral nerve regeneration were evaluated by neural electrophysiology, the recovery rate of triceps surae muscular tension and weight and histological assessment 16 weeks after operation. Results All the animals survived till the end of experiment. The length of nerve regeneration was measured at 2 weeks after transplantation. The regeneration nerve of group A was longer than that of other groups （P 〈 0.05）, group B longer than groups C and D （P 〈 0.05）, and there were no difference between group C and group D （P 〉 0.05）. At 16 weeks after operation, the recovery rates of nerve conduction velocity of groups A and B （73.37%± 7.82% and 70.39% ± 8.45%） were larger than that of groups C and D （53.51% ±6.31% and 55.28% ±5.37%） （P 〈 0.05）. The recovery rates of the triceps surae muscular tension in group A （85.33%± 5.59%） were larger than that in groups B, C and D （69.79%±5.31%, 64.46% ± 8.49% and 63.35%±6.40%） （P 〈 0.05）. There were no significant differences among g

关 键 词：神经再生 神经生长因子 缓释 纤维蛋白胶 去细胞神经 大鼠 

分 类 号：R651.3[医药卫生—外科学]