雷公藤体细胞胚发生及其植株再生  

Somatic Embryogenesis and Plantlet Regeneration of Tripterygium wilfordii

作　　者：李琰[1,2] 崔宏安[2] 冯俊涛[1] 丁虹茹[2] 易晓华[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心陕西省生物农药工程技术研究中心,杨凌712100 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《林业科学》2008年第10期148-152,176,共6页Scientia Silvae Sinicae

基　　金：国家"十五"攻关项目(2002BA516A04)

摘　　要：The callus of Tripterygium wilfordii was used to study the effect of basic media,different plant growth regulators and different concentrations of growth regulators on regeneration of T. wilfordii. And the process of the somatic embryogenesis was observed. The results showed that NAA was more effective auxin than 2,4-D in inducing the plantlet regeneration of T. wilfordii. Cytokinins could promote the callus differentiation,for which 6-BA was more effective than KT. The combination of NAA and 6-BA encouraged more regeneration than each of them used alone. Among the 6 media supplied,MS and B5 brought about 100% regeneration rate for callus. On MS medium supplemented with 0.1 mg·L -1 NAA+1.0 mg·L -1 6-BA,the callus had 100% regeneration rate,and each callus had relatively more shoots on average than other combinations. Histological observation showed that a somatic embryogenesis of T. wilfordii originated from a single cell of the internal of callus and its development procedure was similar to that of a zygotic embryo.The callus of Tripterygium wilfordii was used to study the effect of basic media, different plant growth regulators and different concentrations of growth regulators on regeneration of T. wilfordii. And the process of the somatic embryogenesis was observed. The results showed that NAA was more effective auxin than 2,4-D in inducing the plantlet regeneration of T. wilfordii. Cytokinins could promote the callus differentiation, for which 6-BA was more effective than KT. The combination of NAA and 6-BA encouraged more regeneration than each of them used alone. Among the 6 media supplied, MS and Bs brought about 100 % regeneration rate for callus. On MS medium supplemented with 0.1 mg·L^- 1 NAA ＋ 1.0 mg· L^- 1 6-BA, the callus had 100% regeneration rate, and each callus had relatively more shoots on average than other combinations. Histological observation showed that a somatic embryogenesis of T. wilfordii originated from a single cell of the internal of callus and its development procedure was similar to that of a zygotic embryo.

关 键 词：雷公藤 愈伤组织 体细胞胚发生 植株再生 

分 类 号：S722.37[农业科学—林木遗传育种] Q943.1[农业科学—林学]