东北地区部分大型藓类植物遗传多样性的RAPD研究  

Genetic Diversity of Part of Large Bryophyte in Northeast Region Using RAPD

作　　者：李晶[1] 沙伟[2] 

机构地区：[1]黑龙江生物科技职业学院生物工程系,哈尔滨150025 [2]齐齐哈尔大学生命科学与工程学院生物系,齐齐哈尔161006

出　　处：《植物研究》2008年第6期689-692,共4页Bulletin of Botanical Research

基　　金：国家自然科学基金资助项目(No.30170076)

摘　　要：采用RAPD标记构建了东北地区20种大型藓类植物的系统关系。从200个随机引物中筛选出清晰且多态性高的7个引物,共产生了82条DNA片段,其中79条谱带具有遗传多态性,约占96.34%,平均每个引物扩增的DNA片段数为11.71条。采用NTSYS-pc数据分析软件,计算Nei氏相似性系数,建立了UPGMA聚类图。结果表明:以遗传相似性系数0.27为界限划分,可将20种苔藓植物分为二大类,即泥炭藓类和真藓类。以遗传相似性系数0.42为界限划分,可将20种苔藓植物划分为四大类群,即泥炭藓类、顶蒴单齿亚类、顶蒴双齿亚类和侧蒴双齿类。从整个聚类图可以看出,泥炭藓类是较原始的类群,而金发藓类是较进化的类群,这一结果和大多数苔藓植物经典分类系统相同。The systemtic relationship of 20 species large bryophyte were generated using RAPD method. Seven primers were screened from 200 random primers, and a total of 82 DNA fragments were amplified, 79（96.34% ）of which were polymorphic. The average number of DNA band produced by each primer was 11.71. Neis similarity coefficients were calculated with the software of NTSYS-pc version and den- drogram of 20 species bryophyte was constructed using UPGMA. 20 kinds of bryophyte were divided into two genus at 0.27 genetic similar coefficient. They were Sphagnum and Bryum. 20 kinds of bryophyte may be divided into four genus at 0.42 genetic similar coefficient. They were Sphagnum including 1 spe- cies, Acrocarpi-Haplolepodeae including 2 species, Acrocapi-Diplolepideae including 6 species and Pleurocarpi-Diplolepideae including 10 speices. Bryiidae were divided three clusters. Sphagnum was a primi- tive cluster,Polytrichum was a evolutive cluster. The result was in accordance to most of the classified system of the bryophyte classical taxology.

关 键 词：苔藓植物 系统关系 遗传相似性系数 

分 类 号：Q949[生物学—植物学]