桔梗雄性不育种质JXB-1的发现与鉴定  

Discovery and Identification of Male Sterility Platycodon grandiflorum Germplasm JXB-1

作　　者：吴基日[1] 严一字[1] 全雪丽[1] 朴锦[1] 吴松权[1] 

机构地区：[1]延边大学农学院,龙井133400

出　　处：《植物研究》2008年第6期716-719,共4页Bulletin of Botanical Research

基　　金：国家自然科学基金资助项目(30660016)

摘　　要：2006年在龙井市当地采集的3年生野生桔梗种源圃中发现雄性不育种质JXB-1个体,本研究对其生殖发育进行了进一步的实验验证。结果表明:JXB-1个体花的外观与正常的花比较无明显差异,但花药皱瘪;花药开裂,但无花粉粒散出,强行挤出来的花粉粒多数呈不规则型且无活力;套袋自交座果率为零,与30份不同桔梗种源杂交座果率为100%;经花药发育及小孢子发生过程观察,初步认为JXB-1花粉的败育主要发生在小孢子发育时期,JXB-1的绒毡层细胞过早解体,因而小孢子发育中途停止是败育的主要原因。用组织培养方法进行无性繁殖的JXB-1后代全部表现为雄性不育。初步研究结果表明JXB-1确属雄性不育,与其他种源有高度亲和力,可作为优良的育种材料。A male sterile germplasm JXB-1 was discovered at three-year-old wild Platycodon grandiflorum germplasms area in Longjing city in 2006 and its development and reproduction were further studied. The results showed that there was no obvious difference in flower＇ s outward appearance between JXB-1 and normal plant, but JXB-1 had shriveled and cracked anther with no microspore, forced anther with irregular and no activities, its self-pollination rate was zero and crosspollination rate was 100%. Anther and microsporogenesis observations showed that JXB-1 anther abortion mainly took place at tetrad stage because small spore growth was stopped for tapetum ceils premature disintegration. Vegetative propagations of tissue culture for JXB-1 was all male sterile. It was prelitninarily shown that JXB-1 was a male sterility germplasm, had high affinity with other germplasms and can be used as fine breeding material.

关 键 词：桔梗 雄性不育 种质个体 生殖发育 

分 类 号：Q943[生物学—植物学]