金边瑞香浸膏对小鼠血清IFN-γ、IL-4、IL-18表达的影响  

Effect of Daphne Odora Var.Marginata on Expressions of IFN-γ,IL-4,and IL-18 in Mice

作　　者：况南珍[1] 傅颖媛[1] 徐静[2] 曾小平[1] 陈开森[2] 

机构地区：[1]南昌大学基础医学院免疫学教研室,南昌330006 [2]南昌大学第一附属医院检验科,南昌330006

出　　处：《实用临床医学（江西）》2008年第10期1-2,6,共3页Practical Clinical Medicine

摘　　要：目的观察金边瑞香(daphne odora var.marginata,DOVM)浸膏对小鼠血清干扰素γ(IFN-γ)、白细胞介素4(IL-4)、白细胞介素18(IL-18)表达的影响。方法选用昆明系小鼠60只,随机分为生理盐水组(对照组)和1、2、4、8、16 g/(kg.d)DOVM浸膏组共6组,每组10只。DOVM浸膏不同浓度组分别灌胃给药0.02 mL/g,1次/d,连续14 d;生理盐水组给予生理盐水灌胃0.02 mL/g,1次/d,连续14 d。常规分离血清后,采用生物素双抗体ELISA夹心法(ABC-ELISA)测定各组小鼠血清IFN-γ、IL-4、IL-18的水平。结果1 g/(kg.d)DOVM浸膏组血清IL-4水平明显高于生理盐水组(P<0.05)。生理盐水组与DOVM浸膏不同浓度组血清IFN-γ、IL-18水平比较差异均无统计学意义(P均>0.05),DOVM浸膏不同浓度组间血清IFN-γ、IL-18水平比较差异也均无统计学意义(P均>0.05)。结论1 g/(kg.d)DOVM浸膏对小鼠IL-4的表达有一定促进作用。Objective To observe the effect of daphne odora var. marginata（DOVM） on expressions of IFN-γ（Interferon γ）,IL-4（Interleukin-4）, and IL-18（Interleukin-18） in mice. Methods Sixty kunming mice were randomized into normal sodium saline group（control group） and 1,2,4, 8,16 g/（kg · d） of DOVM groups with 10 mice in each group. Each mouse of DOVM groups was perfused with 0.02 mL/g drug of different concentration and each mouse of normal sodium saline group was perfused with 0.02 mL/g normal sodium saline one time per day for 14 days. To determine level of IFN-γ, IL-4, and IL-18 in mice of each groups were measured by ABC-ELISA. Results The level of IL-4 of 1 g/（kg · d） of DOVM group was higher than that in control group （P〈0.05）. The different of levels of IFN-γ and IL-18 between control group and DOVM different concentration groups was no statistically significant（all P〉0.05）. And no statistically signif- icant was found on the levels of IFN-γ, and IL-18 in DOVM different concentration groups, too （all P〉0.05）. Conclusion 1 g/（kg · d） of DOVM has a remarkable improving effect of IL-4 expressions in mice.

关 键 词：金边瑞香 IFN-Γ IL-4 IL-18 动物 实验 小鼠 

分 类 号：R285.5[医药卫生—中药学] Q786[医药卫生—中医学]