丹参总酮对大鼠在体胃肠道的吸收动力学  

Absorption kinetics of total tanshinones in rats' gastrointestinal tract in situ

作　　者：胡晓丹[1] 赵秀丽[1] 高春凤[1] 张皊皊 陈大为[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2008年第11期856-859,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家“十一五”科技支撑计划资助项目(2006BAI09B08-04)

摘　　要：目的研究丹参总酮在大鼠胃、十二指肠、回肠、空肠、结肠的吸收情况。方法以丹参酮ⅡA(TSIIA)为检测丹参总酮的指标性成分。采用大鼠在体胃肠吸收回流实验装置,利用UV双波长法测定肠循环液中TSIIA及酚红的含量。结果丹参总酮吸收速率ka随浓度的增加有显著下降趋势,吸收半衰期t1/2延长。结论丹参总酮在大鼠肠道的吸收存在饱和现象,提示丹参总酮在机体内的转运机制可能为主动转运或促进扩散。胃内的吸收机制为被动扩散,且丹参总酮最佳吸收部位为结肠。Objective To study the absorption of total tanshinones in rats＇ stomach, duodenum, jejunum, ileum and colon. Methods Tanshinone Ⅱ A（TSⅡA） was used as index component to determine total tanshinones. The in situ perfusion method in rats was employed to investigate the intestinal absorption, The con- centration of TSIIA and phenol red in the solution of in situ intestines were determined by UV dual-wave- length spectrophotometry. Results Various dosages of total tanshinones were administrated. The results indicated that the absorption rate ka decreased slightly, absorption half life time t 1/2 increased gradually with the increase of drug concentration. Conclusions The saturation phenomenon is existed in total tanshinones absorption, which indicates that the transport mechanism of total tansbinones in vivo is similar to active transport or facilitated diffusion. The absorption mechanism in stomach may be passive diffusion. The results also show that the absorption rate in colon is the highest for the passive diffusion of total tanshiones.

关 键 词：丹参总酮 丹参酮IIA 紫外分光光度 双波长法 在体胃肠道吸收 

分 类 号：R94[医药卫生—药剂学]