RP-HPLC法测定甘草中甘草酸差向异构体的含量  

Simultaneous determination of epimer of glycyrrhizic acid in Radix Glycyrrhizae by RP-HPLC

作　　者：郭薇[1] 陈晓辉[1] 景春杰[1] 林景瑞[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2008年第11期892-896,共5页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省教育厅科学技术研究攻关计划(GrantNo.2004D268)

摘　　要：目的建立RP-HPLC法同时测定甘草药材中甘草酸差向异构体的含量。方法采用Hypersil C18（250mm×4．6mm，5μm）柱，以甲醇-体积分数为1％的醋酸溶液（体积比为56：44）为流动相，流速为1．0mL·min^-1，检测波长为250nm，柱温为35℃。结果α-甘草酸和β-甘草酸的线性范围分别为0．010～0．209g·L^-1（r=0．9995）和0．052～1．034g·L^-1（r=0．9996）。2种成分的平均加样回收率（n=9）分别为99．7％和99．4％。结论该方法可用于甘草药材中甘草酸差向异构体的同时含量测定。Objective To develop an RP-HPLC for simultaneous determination of the epimers of glycyrrhizic acid in Radix Glycyrrhizae. Methods The separation was performed on a Hypersil C18 column （250 mm× 4.6 mm, 5 μm）, with methanol-1% acetic acid （V：V = 56：44） as mobile phase and the flow rate was at 1.0 mL·min^-1. The detective wavelength was set at 250 nm and the column temperature was 35℃ Results The liner ranges of α-glycyrrhizic acid and β-glycyrrhizic acid were 0.01- 0.20 g·L^-1（r=0.999 5） and 0.05- 1.00 g·L^-1（r=0.999 6）. The average recoveries（n = 9） of the two compontents were 99.7 % and 99.4 %. Conclusions The method is simple, accurate and reproducible. It can be applied for the simulta-neous determination of the epimers of glycyrrhizic acid of Radix Glycyrrhizae.

关 键 词：反相高效液相色谱法 甘草酸 差向异构体 甘草 

分 类 号：R917[医药卫生—药物分析学]