纳米铂颗粒修饰薄膜金电极的新型葡萄糖传感器研究  

A Novel Glucose Biosensor Based on Thin-film Gold Electrode Modified Nanoparticles

作　　者：姜利英[1] 蔡新霞[2,3] 刘红敏[2,3] 刘春秀[2,3] 郭宗慧[2,3] 

机构地区：[1]郑州轻工业学院电气信息工程学院,郑州450002 [2]中国科学院电子学研究所传感技术国家重点实验室,北京100080 [3]中国科学院研究生院,北京100080

出　　处：《分析化学》2008年第11期1563-1566,共4页Chinese Journal of Analytical Chemistry

基　　金：国家高技术研究发展计划(No.2005AA404220);国家自然科学基金(No.60576049);郑州轻工业学院博士科研基金(No.2007BSJJ002)资助项目

摘　　要：在没有引入电子媒介体条件下,为了提高传感器的响应灵敏度,降低工作电位,利用电化学沉积法在薄膜金电极表面修饰纳米铂颗粒,并通过戊二醛固定酶的方法制备了一种新型生物传感器。研究了在薄膜金电极上修饰纳米铂颗粒前后传感器对低浓度葡萄糖的响应影响。结果表明,纳米铂颗粒修饰后所制备的葡萄糖传感器工作电位下降为0.4 V,测定葡萄糖的检出限从100μmol/L下降到10μmol/L。传感器对10-1300μmol/L低浓度葡萄糖的响应灵敏度为50.8 nA/（cm2μmol/L）;响应时间30 s;r为0.9974;传感器精密度为2.1%,并具有较好的稳定性。To improve the sensitivity and lower the working potential of the biosensor under the condition of no mediator, a new biosensor is introduced and the glucose oxidase is immobilized on the surface of the modified electrode by glutaraldehyde cross-linking. The nano-Pt particles are deposited on the surface of the electrode fabricated by thin-film technology. The analytical characteristics of the biosensor for low concentration are studied at the platinum nanoparticles and ordinary thin-film electrode. The results show the detection limit of the glucose bioseuser decreases from 100 to 10 μ moL/L and the working potential is 0. 4 V. The result exhibits analytical sensitivity of 50.8 nA/（ cm^2 μ mol/L） in the relevant concentration range of 0 - 1300 μ mol/L at the response time 30 s. The RSD is 2.1% The biosensor also indicates good stability.

关 键 词：纳米铂颗粒 薄膜金电极 葡萄糖传感器 丝印参比电极 化学修饰电极 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]