以发光二极管为光源的毛细管电泳芯片-光纤耦合荧光检测微流控分析系统  

Optical Fiber Coupling of Light-Emitting Diode Induced Fluorescence Detection for Microchip Electrophoresis

作　　者：李海芳[1] 陈志锋[2] 林金明[1] 

机构地区：[1]清华大学化学系,北京100084 [2]中国检验检疫科学研究院,北京100123

出　　处：《分析化学》2008年第11期1587-1591,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.30772006,20605018);北京市自然科学基金(No.2072018)资助项目

摘　　要：自行设计组装了简单小型化的芯片毛细管电泳-光纤耦合激光诱导荧光检测系统。以蓝色发光二极管为光源,借助光纤来传导激发光,并通过在芯片上加工与分离通道相垂直的光纤定位通道,实现了激发光在芯片上检测点的准确定位。当光纤定位通道末端与分离通道之间的距离为190μm时,激发光在检测点的光斑直径测定值仅为185μm。以荧光素为标准样品考察了该检测系统的各性能参数,连续5次进样,电泳分离的峰面积标准偏差(RSDs)为6.8%,荧光素的检出限为6μmol/L。说明该检测系统具有低噪音和重现性好等优点。利用该检测系统,快速分离了异硫氰酸荧光素标记的儿茶酚胺样品。A simple and disposable optical fiber coupling of laser induced fluorescence detector for microchip electrophoresis was constructed. On the glass chip, an extra insertion channel for position of the optical fiber, which was perpendicular and extremely close to the separation channel, was directly fabricated by step-etching procedure. A blue light-emitting diode （LED） was used as excitation source and an optical fiber was employed to collect and transmit the excitation light to the detection point. When the distance between the separation channel and the end of the insersion channel was controlled to be as short as 190 μm, the excitation spot diameter was measured to be 185 μm. Fluorescein was taken as model analyte to evaluate the performances of this simple detection system. The detection limit for fluorescein was as low as 6 μmoL/L and the relative standard deviation （RSDs） of peak area was 6.8% （n = 5 ）. The suitability of the detection system for fluorescence detection was demonstrated by rapid baseline separation of fluorescein isothiocyanate （FITC） labeled catecholamines.

关 键 词：芯片毛细管电泳 激光诱导荧光 光纤 发光二极管 

分 类 号：O658.9[理学—分析化学]